• Product nameAnti-ZSCAN4 antibody
    See all ZSCAN4 primary antibodies
  • Description
    Rabbit polyclonal to ZSCAN4
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human ZSCAN4.

    (Peptide available as ab109888.)

  • Positive control
    • This antibody gave a positive signal in Human kidney tissue lysate as well as the following whole cell lysates: HEK293; A498; HuES7.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab97748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 49 kDa).


  • FunctionEmbryonic stem (ES) cell-specific transcription factor required to regulate ES cell pluripotency. Binds telomeres and plays a key role in genomic stability in ES cells by regulating telomere elongation. Acts as an activator of spontaneous telomere sister chromatid exchange (T-SCE) and telomere elongation in undifferentiated ES cells.
  • Sequence similaritiesContains 4 C2H2-type zinc fingers.
    Contains 1 SCAN box domain.
  • Cellular localizationNucleus. Chromosome > telomere.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ35105 antibody
    • MGC126787 antibody
    • MGC126789 antibody
    • Zinc finger and SCAN domain containing 4 antibody
    • Zinc finger and SCAN domain containing protein 4 antibody
    • Zinc finger and SCAN domain-containing protein 4 antibody
    • Zinc finger protein 494 antibody
    • ZNF 494 antibody
    • ZNF494 antibody
    • ZSCA4_HUMAN antibody
    • ZSCAN4 antibody
    see all

Anti-ZSCAN4 antibody images

  • All lanes : Anti-ZSCAN4 antibody (ab97748) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
    Lane 4 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

References for Anti-ZSCAN4 antibody (ab97748)

ab97748 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (SHEF3 hESC)
Loading amount 20 µg
Specification SHEF3 hESC
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted Mar 08 2012