The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 49 kDa).
Embryonic stem (ES) cell-specific transcription factor required to regulate ES cell pluripotency. Binds telomeres and plays a key role in genomic stability in ES cells by regulating telomere elongation. Acts as an activator of spontaneous telomere sister chromatid exchange (T-SCE) and telomere elongation in undifferentiated ES cells.
The predicted molecular weight of ZSCAN4 is 49 kDa (SwissProt), however we expect to observe a banding pattern at 58 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.