Overview

  • Product name
  • Description
    Rabbit polyclonal to ZSCAN4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human ZSCAN4.

    (Peptide available as ab109888.)

  • Positive control
    • This antibody gave a positive signal in Human kidney tissue lysate as well as the following whole cell lysates: HEK293; A498; HuES7.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 49 kDa).

Target

  • Function
    Embryonic stem (ES) cell-specific transcription factor required to regulate ES cell pluripotency. Binds telomeres and plays a key role in genomic stability in ES cells by regulating telomere elongation. Acts as an activator of spontaneous telomere sister chromatid exchange (T-SCE) and telomere elongation in undifferentiated ES cells.
  • Sequence similarities
    Contains 4 C2H2-type zinc fingers.
    Contains 1 SCAN box domain.
  • Cellular localization
    Nucleus. Chromosome > telomere.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ35105 antibody
    • MGC126787 antibody
    • MGC126789 antibody
    • Zinc finger and SCAN domain containing 4 antibody
    • Zinc finger and SCAN domain containing protein 4 antibody
    • Zinc finger and SCAN domain-containing protein 4 antibody
    • Zinc finger protein 494 antibody
    • ZNF 494 antibody
    • ZNF494 antibody
    • ZSCA4_HUMAN antibody
    • ZSCAN4 antibody
    see all

Images

  • All lanes : Anti-ZSCAN4 antibody (ab97748) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
    Lane 4 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 49 kDa
    Observed band size: 58 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds


    The predicted molecular weight of ZSCAN4 is 49 kDa (SwissProt), however we expect to observe a banding pattern at 58 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

References

ab97748 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (SHEF3 hESC)
Loading amount
20 µg
Specification
SHEF3 hESC
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Mar 08 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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