Overview

  • Product name
    Anti-ZSWIM6 antibody
  • Description
    Rabbit polyclonal to ZSWIM6
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen, corresponding to amino acids 634-696 (DPVGTLFSSL MEACRIDDEN LSGFSDFTEN MGQCKSLEYQ HLPAHKFLEE GESYLTLAVE VAL) of Human ZSWIM6.

  • Positive control
    • Human smooth muscle tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab122301 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122301, at 1/75, staining ZSWIM6 in paraffin embedded Human smooth muscle tissue by Immunohistochemistry.

References

This product has been referenced in:
  • Smith JD  et al. Exome sequencing identifies a recurrent de novo ZSWIM6 mutation associated with acromelic frontonasal dysostosis. Am J Hum Genet 95:235-40 (2014). Read more (PubMed: 25105228) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (brain)
Permeabilization
Yes - 0.2% triton X-100/0.1M PBS, 15-20mins
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Miss. wan ting lin

Verified customer

Submitted Mar 31 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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