Anti-ZW10 antibody [3363C4a] (ab53676)
Key features and details
- Mouse monoclonal [3363C4a] to ZW10
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-ZW10 antibody [3363C4a]
See all ZW10 primary antibodies -
Description
Mouse monoclonal [3363C4a] to ZW10 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
ZW10 recombinant fragment, internal sequence, (Human).
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General notes
The antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
3363C4a -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53676 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 89 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 89 kDa. |
Target
- Information by UniProt
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Alternative names
- Centromere/kinetochore protein zw10 antibody
- Centromere/kinetochore protein zw10 homolog antibody
- HZW 10 antibody
see all
Images
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Anti-ZW10 antibody [3363C4a] (ab53676) + Immunizing recombinant ZW10 fragment
Predicted band size: 89 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
Datasheets and documents
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Datasheet download
References (3)
ab53676 has been referenced in 3 publications.
- Conti D et al. Kinetochores attached to microtubule-ends are stabilised by Astrin bound PP1 to ensure proper chromosome segregation. Elife 8:N/A (2019). PubMed: 31808746
- Brownlow N et al. Mitotic catenation is monitored and resolved by a PKCe-regulated pathway. Nat Commun 5:5685 (2014). IF . PubMed: 25483024
- Ryu H & Azuma Y Rod/Zw10 complex is required for PIASy-dependent centromeric SUMOylation. J Biol Chem 285:32576-85 (2010). PubMed: 20696768