Overview

  • Product nameAnti-ZWILCH antibody
    See all ZWILCH primary antibodies
  • Description
    Rabbit polyclonal to ZWILCH
  • Tested applicationsSuitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 541-591 of Human ZWILCH using the numbering given in entry NP_060445.3 (GeneID 55055).

  • Positive control
    • HeLa cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab105117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 2 - 5 µg/ml.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionEssential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex (PubMed:15824131).
    • Sequence similaritiesBelongs to the ZWILCH family.
    • Cellular localizationChromosome, centromere, kinetochore.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ10036 antibody
      • FLJ16343 antibody
      • homolog of Drosophila Zwilch antibody
      • hZwilch antibody
      • KNTC1AP antibody
      • MGC111034 antibody
      • Protein zwilch homolog antibody
      • ZWILC_HUMAN antibody
      • zwilch antibody
      • zwilch kinetochore protein antibody
      • ZWILCH protein antibody
      • Zwilch, kinetochore associated, homolog antibody
      see all

    Anti-ZWILCH antibody images

    • HeLa cell lysate immunoprecipitated using ab105117 (Left Lane) at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) for the analysis of ZWILCH expression. Subsequent WB detection was performed using ab105117 at 1 µg/ml (as the sample is enriched by IP). Rightr Lane represents IP with IgG control. Exposure time: 10 seconds.

    References for Anti-ZWILCH antibody (ab105117)

    ab105117 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"