Overview

  • Product name
  • Description
    Rabbit polyclonal to ZWILCH
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 270-511 of Human ZWILCH (UniProt Q9H900).

  • Positive control
    • NT2D1, IMR32, U87-MG and MCF7 cell lysates; A431 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 67, 54 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex (PubMed:15824131).
  • Sequence similarities
    Belongs to the ZWILCH family.
  • Cellular localization
    Chromosome, centromere, kinetochore.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ10036 antibody
    • FLJ16343 antibody
    • homolog of Drosophila Zwilch antibody
    • hZwilch antibody
    • KNTC1AP antibody
    • MGC111034 antibody
    • Protein zwilch homolog antibody
    • ZWILC_HUMAN antibody
    • zwilch antibody
    • zwilch kinetochore protein antibody
    • ZWILCH protein antibody
    • Zwilch, kinetochore associated, homolog antibody
    see all

Images

  • Anti-ZWILCH antibody (ab154534) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg

    Predicted band size: 67, 54 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling ZWILCH with ab154534 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.

References

ab154534 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab154534.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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