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Rabbit polyclonal to ZWILCH
Recombinant fragment corresponding to a region within amino acids 270-511 of Human ZWILCH (UniProt Q9H900).
NT2D1, IMR32, U87-MG and MCF7 cell lysates; A431 cells.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 67, 54 kDa.
1/100 - 1/1000.
Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex (PubMed:15824131).
Belongs to the ZWILCH family.
Chromosome, centromere, kinetochore.
Information by UniProt
homolog of Drosophila Zwilch antibody
Western blot - Anti-ZWILCH antibody (ab154534)
Anti-ZWILCH antibody (ab154534) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg
Predicted band size: 67, 54 kDa 7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-ZWILCH antibody (ab154534)
Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling ZWILCH with ab154534 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"