Overview

  • Product name
  • Description
    Rabbit polyclonal to ZWINT
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region between residue 225 and the C terminus (residue 277) of human ZWINT (NP_008988.2).

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab71982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 31 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/50 - 1/250.

Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.

Target

  • Function
    Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase.
  • Cellular localization
    Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase.
  • Information by UniProt
  • Database links
  • Alternative names
    • Human ZW10 interacting protein 1 antibody
    • HZwint 1 antibody
    • HZwint1 antibody
    • KNTC 2 AP antibody
    • KNTC2AP antibody
    • MGC 117174 antibody
    • MGC117174 antibody
    • ZW10 interacting kinetochore protein antibody
    • ZW10 interacting protein 1 antibody
    • ZW10 interactor antibody
    • ZW10 interactor, kinetochore protein antibody
    • ZW10-interacting protein 1 antibody
    • ZWINT 1 antibody
    • ZWINT antibody
    • Zwint-1 antibody
    • ZWINT_HUMAN antibody
    • ZWINT1 antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of formaldelyde-fixed HeLa cells labelling ZWINT with ab71982 at at 1/200 (1µg/ml). A FITC-conjugated goat anti-rabbit IgG (1/100) was used as the secondary antibody.
  • All lanes : Anti-ZWINT antibody (ab71982) at 0.04 µg

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 31 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Detection of ZWINT by Western Blot of Immunprecipitate.
    ab71982 at 1µg/ml staining ZWINT in HeLa whole cell lysate immunoprecipitated using ab71982 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab71982 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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