Overview

  • Product name
  • Description
    Rabbit polyclonal to ZWINT
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Macaque monkey
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 225-277 (

    PEAEAEAENLPDDKPQQPTRPQEQSTGDTMGRDPGVSFKAVGLQPAGDVN LP

    ) of Human ZWINT (NP_008988.2)

  • Positive control
    • Human testis tissue. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab84367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/500. Fix with PFA do not fix with acetone.
IHC-P 1/100 - 1/500.

Target

  • Function
    Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase.
  • Cellular localization
    Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase.
  • Information by UniProt
  • Database links
  • Alternative names
    • Human ZW10 interacting protein 1 antibody
    • HZwint 1 antibody
    • HZwint1 antibody
    • KNTC 2 AP antibody
    • KNTC2AP antibody
    • MGC 117174 antibody
    • MGC117174 antibody
    • ZW10 interacting kinetochore protein antibody
    • ZW10 interacting protein 1 antibody
    • ZW10 interactor antibody
    • ZW10 interactor, kinetochore protein antibody
    • ZW10-interacting protein 1 antibody
    • ZWINT 1 antibody
    • ZWINT antibody
    • Zwint-1 antibody
    • ZWINT_HUMAN antibody
    • ZWINT1 antibody
    see all

Images

  • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human testis tissue using  ab84367 at 1/250, followed by DAB staining.

  • ab84367 at 1/250 dillution staining ZWINT in NBF-fixed asynchronous human Hela cells by Immunocytochemistry / Immunofluorescence.

References

This product has been referenced in:
  • Faisal A  et al. Characterisation of CCT271850, a selective, oral and potent MPS1 inhibitor, used to directly measure in vivo MPS1 inhibition vs therapeutic efficacy. Br J Cancer 116:1166-1176 (2017). Read more (PubMed: 28334731) »
  • Brownlow N  et al. Mitotic catenation is monitored and resolved by a PKCe-regulated pathway. Nat Commun 5:5685 (2014). IF . Read more (PubMed: 25483024) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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