Recombinant
RabMAb

Anti-ZWINT antibody [EPR10905] (ab174266)

Overview

  • Product name
    Anti-ZWINT antibody [EPR10905]
    See all ZWINT primary antibodies
  • Description
    Rabbit monoclonal [EPR10905] to ZWINT
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ZWINT aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: O95229

  • Positive control
    • MOLT4, A431, Raji and HeLa cell lysates. Immunoprecipitation pellet from Molt-4 cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab174266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase.
    • Cellular localization
      Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase.
    • Information by UniProt
    • Database links
    • Alternative names
      • Human ZW10 interacting protein 1 antibody
      • HZwint 1 antibody
      • HZwint1 antibody
      • KNTC 2 AP antibody
      • KNTC2AP antibody
      • MGC 117174 antibody
      • MGC117174 antibody
      • ZW10 interacting kinetochore protein antibody
      • ZW10 interacting protein 1 antibody
      • ZW10 interactor antibody
      • ZW10 interactor, kinetochore protein antibody
      • ZW10-interacting protein 1 antibody
      • ZWINT 1 antibody
      • ZWINT antibody
      • Zwint-1 antibody
      • ZWINT_HUMAN antibody
      • ZWINT1 antibody
      see all

    Images

    • All lanes : Anti-ZWINT antibody [EPR10905] (ab174266) at 1/1000 dilution

      Lane 1 : MOLT4 cell lysates
      Lane 2 : A431 cell lysates
      Lane 3 : Raji cell lysates
      Lane 4 : HeLa cell lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 31 kDa
    • Western blot analysis on immunoprecipitation pellet from Molt-4 cell lysate using ab174266 at a 1/10 dilution.

    References

    ab174266 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab174266.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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