Overview

  • Product name
  • Description
    Rabbit polyclonal to ZYG11B
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: DATGVNADIT ITDIISGLGS NKWIQQNLQC LVLNSLTLSL EDPYERCFSR LSGLRALSIT NVLFYNEDLA EVASLPRLES LDISNTSI, corresponding to amino acids 111-198 of Human ZYG11B Isoform 1 (Q9C0D3).

  • Positive control
    • Human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Function
    Probably acts as target recruitment subunit in the E3 ubiquitin ligase complex ZYG11B-CUL2-Elongin BC.
  • Sequence similarities
    Belongs to the zyg-11 family.
    Contains 2 ARM repeats.
    Contains 3 LRR (leucine-rich) repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA1730 antibody
    • Protein zyg-11 homolog B antibody
    • ZY11B_HUMAN antibody
    • zyg-11 family member B, cell cycle regulator antibody
    • ZYG11 antibody
    • Zyg11b antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoskeleton (intermediate filaments). Recommended concentration of ab121975 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121975 staining ZIG11B in formalin-fixed, paraffin-embedded Human kidney tissue by immunohistochemistry.

References

ab121975 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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