Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab11518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 82-84 kDa (predicted molecular weight: 65 kDa).
ICC/IF Use at an assay dependent dilution.

Target

  • RelevanceZyxin binds alpha-actinin and the CRP proteins and is implicated in the regulation and modulation of actin filaments organization, cell adhesion, cell motility, mitosis, and signal transduction. Zyxin may shuttle between focal adhesion plaques and the nucleus. It has been suggested that zyxin is involved in gene transcription by its association with transcription factors in the nucleus.
  • Cellular localizationCytoplasmic, cytoskeletal and Nuclear.
  • Database links
  • Alternative names
    • ESP 2 antibody
    • ESP2 antibody
    • HED 2 antibody
    • HED2 antibody
    • ZYX antibody
    • ZYX protein antibody
    • Zyxin 2 antibody
    • Zyxin2 antibody
    see all

References for Anti-Zyxin antibody (ab11518)

This product has been referenced in:
  • Mise N  et al. Zyxin Is a Transforming Growth Factor-ß (TGF-ß)/Smad3 Target Gene That Regulates Lung Cancer Cell Motility via Integrin a5ß1. J Biol Chem 287:31393-405 (2012). WB . Read more (PubMed: 22778267) »
  • Jarjour AA  et al. Autocrine netrin function inhibits glioma cell motility and promotes focal adhesion formation. PLoS One 6:e25408 (2011). ICC/IF ; Human . Read more (PubMed: 21980448) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Arterial Endothelial Cells)
Specification Arterial Endothelial Cells
Fixative Paraformaldehyde
Permeabilization Yes - 0,1 % Triton X100
Username

Mrs. Elena Astanina

Verified customer

Submitted Apr 15 2008

Thank you for your enquiry and patience. The originator of ab11518 tested the antibody in Western blotting using Hela cell extract and a band at approximately 82-84 kDa was detected. The following reference (the full text article is free) explains more...

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Thank you for your enquiry. I'm still trying to obtain some more specific information about how ab11518 was tested in Western blotting and I'll let you know when I have such info, but our source for this antibody did pass along the following reference ...

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Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with cow has no...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"