Anti-Zyxin antibody (ab71842)
Key features and details
- Rabbit polyclonal to Zyxin
- Suitable for: IP, ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Zyxin antibody
See all Zyxin primary antibodies -
Description
Rabbit polyclonal to Zyxin -
Host species
Rabbit -
Tested applications
Suitable for: IP, ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Dog, Rhesus monkey -
Immunogen
Synthetic peptide corresponding to Human Zyxin aa 450-550 conjugated to keyhole limpet haemocyanin.
(Peptide available asab71841) -
Positive control
- This antibody gave a positive signal in the following Lysates: SK N SH Whole Cell, HeLa Whole Cell, A498 Whole Cell, U-87 MG Whole Cell, DU 145 Whole Cell, Rat Testis Tissue, HCT 116 Whole Cell (data not shown)
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab71842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
Use a concentration of 5 µg/ml.
|
|
ICC/IF | (1) |
Use a concentration of 5 µg/ml.
|
WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 61 kDa).
|
Notes |
---|
IP
Use a concentration of 5 µg/ml. |
ICC/IF
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 61 kDa). |
Target
-
Function
Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression. -
Sequence similarities
Belongs to the zyxin/ajuba family.
Contains 3 LIM zinc-binding domains. -
Cellular localization
Cytoplasm. Cytoplasm, cytoskeleton. Nucleus. Cell junction, focal adhesion. Associates with the actin cytoskeleton near the adhesion plaques. Enters the nucleus in the presence of HESX1. - Information by UniProt
-
Database links
- Entrez Gene: 7791 Human
- Entrez Gene: 22793 Mouse
- Entrez Gene: 114636 Rat
- Entrez Gene: 706407 Rhesus monkey
- Omim: 602002 Human
- SwissProt: Q15942 Human
- SwissProt: Q62523 Mouse
- Unigene: 490415 Human
see all -
Alternative names
- ESP 2 antibody
- ESP2 antibody
- HED 2 antibody
see all
Images
-
All lanes : Anti-Zyxin antibody (ab71842) at 1 µg/ml
Lane 1 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lane 4 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
Lane 5 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate
Lane 6 : Testis (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 48 kDa. We are unsure as to the identity of these extra bands. -
Zyxin was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Zyxin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab71842.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 75kDa; Zyxin -
ICC/IF image of ab71842 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71842, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 5µg/ml, and in 100% Methanol fixed (5 min) Hela, Hek293, HepG2, and MCF-7 cells at 5µg/ml.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (10)
ab71842 has been referenced in 10 publications.
- Tonazzini I et al. Neuronal contact guidance and YAP signaling on ultra-small nanogratings. Sci Rep 10:3742 (2020). PubMed: 32111918
- Butt BG et al. Insights into herpesvirus assembly from the structure of the pUL7:pUL51 complex. Elife 9:N/A (2020). PubMed: 32391791
- Tonazzini I et al. The role of ubiquitin ligase E3A in polarized contact guidance and rescue strategies in UBE3A-deficient hippocampal neurons. Mol Autism 10:41 (2019). PubMed: 31798818
- Sikorski K et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371
- Albecka A et al. Dual Function of the pUL7-pUL51 Tegument Protein Complex in Herpes Simplex Virus 1 Infection. J Virol 91:N/A (2017). ICC ; Human . PubMed: 27852850
- Harrington KM & Clevenger CV Identification of NEK3 Kinase Threonine 165 as a Novel Regulatory Phosphorylation Site That Modulates Focal Adhesion Remodeling Necessary for Breast Cancer Cell Migration. J Biol Chem 291:21388-21406 (2016). PubMed: 27489110
- Van Itallie CM et al. Biotin ligase tagging identifies proteins proximal to E-cadherin, including lipoma preferred partner, a regulator of epithelial cell-cell and cell-substrate adhesion. J Cell Sci 127:885-95 (2014). PubMed: 24338363
- Ketscher A et al. LSD1 controls metastasis of androgen-independent prostate cancer cells through PXN and LPAR6. Oncogenesis 3:e120 (2014). WB, IP ; Human . PubMed: 25285406
- Vyas S et al. A systematic analysis of the PARP protein family identifies new functions critical for cell physiology. Nat Commun 4:2240 (2013). PubMed: 23917125
- Watanabe-Nakayama T et al. Requirement of LIM domains for the transient accumulation of paxillin at damaged stress fibres. Biol Open 2:667-74 (2013). ICC/IF . PubMed: 23862014