Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab50391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 61 kDa.
ELISA Use at an assay dependent concentration.

Array: Use at an assay dependent dilution.

ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    Zyxin binds alpha-actinin and the CRP proteins and is implicated in the regulation and modulation of actin filaments organization, cell adhesion, cell motility, mitosis, and signal transduction. Zyxin may shuttle between focal adhesion plaques and the nucleus. It has been suggested that zyxin is involved in gene transcription by its association with transcription factors in the nucleus.
  • Cellular localization
    Cytoplasmic, cytoskeletal and Nuclear.
  • Database links
  • Alternative names
    • ESP 2 antibody
    • ESP2 antibody
    • HED 2 antibody
    • HED2 antibody
    • ZYX antibody
    • ZYX protein antibody
    • Zyxin 2 antibody
    • Zyxin2 antibody
    see all

Anti-Zyxin antibody [ZOL301] images

  • ab50391 staining rat neuronal progenitor cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.2% Triton X100 for 5 minutes prior to blocking in 10% serum at RT for 1 hour.  The primary antibody was incubated with the sample for 1 hour at 4 µg/ml.  An Alexa Fluor® 488 conjugated goat anti-mouse antibody diluted 1/1000 was used as the secondary.

    See Abreview

References for Anti-Zyxin antibody [ZOL301] (ab50391)

This product has been referenced in:
  • Han X  et al. Zyxin regulates endothelial von Willebrand factor secretion by reorganizing actin filaments around exocytic granules. Nat Commun 8:14639 (2017). Read more (PubMed: 28256511) »
  • Lock FE  et al. Differential regulation of adhesion complex turnover by ROCK1 and ROCK2. PLoS One 7:e31423 (2012). ICC/IF ; Human . Read more (PubMed: 22348083) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (neuronal progenitor cell)
Specification
neuronal progenitor cell
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton X100 for 5 minutes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Anne Chiaramello

Verified customer

Submitted Apr 18 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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