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Rabbit polyclonal to Zyxin (phospho S142)
ab78910 detects endogenous levels of Zyxin only when phosphorylated at serine 142.
Synthetic phosphopeptide derived from human Zyxin around the phosphorylation site of serine 142 (K-V-S
Extracts from Jurkat cells treated with paclitaxel (1uM, 24hours).
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
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Immunogen affinity purified
ab78910 was purified by affinity chromatography using an epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 61 kDa.
Zyxin binds alpha-actinin and the CRP proteins and is implicated in the regulation and modulation of actin filaments organization, cell adhesion, cell motility, mitosis, and signal transduction. Zyxin may shuttle between focal adhesion plaques and the nucleus. It has been suggested that zyxin is involved in gene transcription by its association with transcription factors in the nucleus.
Cytoplasmic, cytoskeletal and Nuclear.
ESP 2 antibody
HED 2 antibody
Western blot - Anti-Zyxin (phospho S142) antibody (ab78910)
All lanes : Anti-Zyxin (phospho S142) antibody (ab78910) at 1/500 dilution Lane 1 : Extracts from Jurkat cells, treated with paclitaxel (1uM, 24hours) Lane 2 : Extracts from Jurkat cells, treated with paclitaxel (1uM, 24hours) with immunising phosphopeptide at 5 µg Lysates/proteins at 5 µg per lane. Predicted band size: 61 kDa Observed band size: 61 kDa
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