Overview

  • Product name
    Anti-Zyxin (phospho S142) antibody
    See all Zyxin primary antibodies
  • Description
    Rabbit polyclonal to Zyxin (phospho S142)
  • Specificity
    ab78910 detects endogenous levels of Zyxin only when phosphorylated at serine 142.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide derived from human Zyxin around the phosphorylation site of serine 142 (K-V-SP-S-I)

  • Positive control
    • Extracts from Jurkat cells treated with paclitaxel (1uM, 24hours).

Properties

Applications

Our Abpromise guarantee covers the use of ab78910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 61 kDa.
ELISA 1/10000.

Target

  • Relevance
    Zyxin binds alpha-actinin and the CRP proteins and is implicated in the regulation and modulation of actin filaments organization, cell adhesion, cell motility, mitosis, and signal transduction. Zyxin may shuttle between focal adhesion plaques and the nucleus. It has been suggested that zyxin is involved in gene transcription by its association with transcription factors in the nucleus.
  • Cellular localization
    Cytoplasmic, cytoskeletal and Nuclear.
  • Database links
  • Alternative names
    • ESP 2 antibody
    • ESP2 antibody
    • HED 2 antibody
    • HED2 antibody
    • ZYX antibody
    • ZYX protein antibody
    • Zyxin 2 antibody
    • Zyxin2 antibody
    see all

Images

  • All lanes : Anti-Zyxin (phospho S142) antibody (ab78910) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells, treated with paclitaxel (1uM, 24hours)
    Lane 2 : Extracts from Jurkat cells, treated with paclitaxel (1uM, 24hours) with immunising phosphopeptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 61 kDa
    Observed band size : 61 kDa

References

This product has been referenced in:
  • Bernusso VA  et al. Imatinib restores VASP activity and its interaction with Zyxin in BCR-ABL leukemic cells. Biochim Biophys Acta 1853:388-95 (2015). Read more (PubMed: 25450971) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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