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Rabbit polyclonal to ZZEF1
antigen sequence, corresponding to amino acids 2493-2568 (DYFFLEVQKR FDGDELTTDE RIRSLAQRWQ PSKSLRLEEQ SAKAVDTDMI ILPCLSRPAR CDQATAESNP VTQKLI) of Human ZZEF1.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Expressed at low levels in cerebellum.
Contains 1 DOC domain.
Contains 1 EF-hand domain.
Contains 2 ZZ-type zinc fingers.
Information by UniProt
- FLJ10821 antibody
- KIAA0399 antibody
- Zinc finger ZZ-type and EF-hand domain-containing protein 1 antibody
Immunocytochemistry/ Immunofluorescence - Anti-ZZEF1 antibody (ab122194)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and mitochondria. Recommended concentration of ab122194 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZZEF1 antibody (ab122194)
ab122194 at 1/400 dilution staining ZZEF1 in Paraffin-embedded Human Testis tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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