Overview

  • Product name
  • Description
    Rabbit polyclonal to ZZEF1
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 2493-2568 (DYFFLEVQKR FDGDELTTDE RIRSLAQRWQ PSKSLRLEEQ SAKAVDTDMI ILPCLSRPAR CDQATAESNP VTQKLI) of Human ZZEF1.

  • Positive control
    • Human testis tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Tissue specificity
    Expressed at low levels in cerebellum.
  • Sequence similarities
    Contains 1 DOC domain.
    Contains 1 EF-hand domain.
    Contains 2 ZZ-type zinc fingers.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ10821 antibody
    • KIAA0399 antibody
    • Zinc finger ZZ-type and EF-hand domain-containing protein 1 antibody
    • Zinc finger, ZZ-type with EF hand domain 1 antibody
    • ZZEF1 antibody
    • ZZEF1_HUMAN antibody
    • ZZZ4 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and mitochondria. Recommended concentration of ab122194 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122194 at 1/400 dilution staining ZZEF1 in Paraffin-embedded Human Testis tissue by Immunohistochemistry.

References

ab122194 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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