Overview

  • Product name
  • Description
    Rabbit polyclonal to ZZEF1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human ZZEF1 aa 525-575. The exact sequence is proprietary. NP_055928.3
    Sequence:

    GKPLQL TLQACDVKGK EDKSGPENLL VEPWTRDGFL TETGKTRAST IFSTG


    Database link: O43149

  • Positive control
    • 293T whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.

ab176594 not performed satisfactorily when used for WB of ZZEF1 in crude preparations (e.g. whole cell lysate), however can be used for WB of enriched (e.g. immunoprecipitated) sources of ZZEF1.

 

  • Application notes
    Is unsuitable for WB.
  • Target

    Images

    • Detection of ZZEF1 by Western Blot of Immunprecipitate.
      ab176594 at 0.4µg/ml labeling ZZEF1 in 293T whole cell lysate immunoprecipitated using ab176594 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

       

    References

    ab176594 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab176594.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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