Anti-ZZEF1 antibody (ab176594)
Key features and details
- Rabbit polyclonal to ZZEF1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZZEF1 antibody
See all ZZEF1 primary antibodies -
Description
Rabbit polyclonal to ZZEF1 -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Human ZZEF1 aa 525-575. The exact sequence is proprietary. NP_055928.3
Sequence:GKPLQL TLQACDVKGK EDKSGPENLL VEPWTRDGFL TETGKTRAST IFSTG
Database link: O43149 -
Positive control
- 293T whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-10 µg/mg of lysate.
ab176594 not performed satisfactorily when used for WB of ZZEF1 in crude preparations (e.g. whole cell lysate), however can be used for WB of enriched (e.g. immunoprecipitated) sources of ZZEF1.
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Notes |
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IP
Use at 2-10 µg/mg of lysate. ab176594 not performed satisfactorily when used for WB of ZZEF1 in crude preparations (e.g. whole cell lysate), however can be used for WB of enriched (e.g. immunoprecipitated) sources of ZZEF1.
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Target
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Tissue specificity
Expressed at low levels in cerebellum. -
Sequence similarities
Contains 1 DOC domain.
Contains 1 EF-hand domain.
Contains 2 ZZ-type zinc fingers. - Information by UniProt
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Database links
- Entrez Gene: 23140 Human
- Entrez Gene: 195018 Mouse
- SwissProt: O43149 Human
- SwissProt: Q5SSH7 Mouse
- Unigene: 277624 Human
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Alternative names
- FLJ10821 antibody
- KIAA0399 antibody
- Zinc finger ZZ-type and EF-hand domain-containing protein 1 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab176594 has been referenced in 1 publication.
- Espinoza S et al. Neuronal surface P antigen (NSPA) modulates postsynaptic NMDAR stability through ubiquitination of tyrosine phosphatase PTPMEG. BMC Biol 18:164 (2020). PubMed: 33158444