Product name(-)-Bicuculline methobromide, GABAA antagonist
Chemical name[S-(R*,S*)]-5-(6,8-Dihydro-8-oxofuro[3,4-e]-1,3-benzodioxol-6-yl)-5,6,7,8-tetrahydro-6,6-dimethyl-1,3-dioxolo[4,5-g]isoquinolinium bromide
Storage instructionsStore at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in water to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Saturation of LTP by nicotine application.
VSD recordings were performed from the granular layer of cerebellar slices (pre-treated with 10 µM bicuculline methobromide) to measure the spatial organisation of the effect of nicotine following TBS.
(Panel A) Images of background epifluorescence in stained cerebellar slices show the granular layer (GRL), Purkinje cell layer (PCL), molecular layer (ML), mossy fibre bundle (mf), and position of the stimulating electrode (yellow dots). Coloured optical maps of granular layer activity evoked by a single mossy fibre pulse are compared before and after the induction of long-term synaptic plasticity by TBS.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab120109 has been referenced in 9 publications.
- Ge Y et al. Clptm1 Limits Forward Trafficking of GABAA Receptors to Scale Inhibitory Synaptic Strength. Neuron 97:596-610.e8 (2018). PubMed: 29395912
- Zhang P et al. Heparan Sulfate Organizes Neuronal Synapses through Neurexin Partnerships. Cell 174:1450-1464.e23 (2018). PubMed: 30100184
- Kosillo P et al. Cortical Control of Striatal Dopamine Transmission via Striatal Cholinergic Interneurons. Cereb Cortex N/A:N/A (2016). PubMed: 27566978
- Klar R et al. Activation of Metabotropic Glutamate Receptor 7 Is Required for Induction of Long-Term Potentiation at SC-CA1 Synapses in the Hippocampus. J Neurosci 35:7600-15 (2015). PubMed: 25972184
- Lalchandani RR & Vicini S Inhibitory collaterals in genetically identified medium spiny neurons in mouse primary corticostriatal cultures. Physiol Rep 1:e00164 (2013). PubMed: 24400165
- Prestori F et al. Gating of long-term potentiation by nicotinic acetylcholine receptors at the cerebellum input stage. PLoS One 8:e64828 (2013). PubMed: 23741401
- Luo R et al. Distinct roles of synaptic and extrasynaptic GABAAreceptors in striatal inhibition dynamics. Front Neural Circuits 7:186 (2013). PubMed: 24324406
- Johnson KA et al. Activation of group II metabotropic glutamate receptors induces long-term depression of excitatory synaptic transmission in the substantia nigra pars reticulata. Neurosci Lett 504:102-6 (2011). PubMed: 21945652
- Takazawa T & MacDermott AB Glycinergic and GABAergic tonic inhibition fine tune inhibitory control in regionally distinct subpopulations of dorsal horn neurons. J Physiol 588:2571-87 (2010). PubMed: 20498232