(-)-Cannabidiol, Natural cannabinoid (ab120448)
Overview
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Product name
(-)-Cannabidiol, Natural cannabinoid -
Description
Common constituent of Cannabis -
Alternative names
- CBD
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Biological description
Common constituent of Cannabis with much lower affinity at CB1 and CB2 receptors (Ki values are 4350 and 2860 nM, respectively) than Delta-9 THC. Elicits anticonvulsant, anticancer, anti-inflammatory and neuroprotective effects. Protects retinal neurons in diabetic models. -
Purity
> 98% -
CAS Number
13956-29-1 -
Chemical structure
Properties
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Chemical name
2-[(1R,6R)-3-Methyl-6-(1-methylethenyl)-2-cyclohexen-1-yl]-5-pentyl-1,3-benzenediol -
Molecular weight
314.47 -
Molecular formula
C21H30O2 -
PubChem identifier
644019 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM and in ethanol to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CC(=C)[C@@H]2CCC(C)=C[C@H]2c1c(O)cc(CCCCC)cc1O -
Source
Synthetic
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Research areas
Applications
Our Abpromise guarantee covers the use of ab120448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies | Use at an assay dependent concentration. |
Images
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Functional Studies - (-)-Cannabidiol, Natural cannabinoid (ab120448)ab109225 staining Nox4 in HeLa cells treated with (-)-cannabidiol (ab120448), by ICC/IF. Increase in Nox4 expression correlates with increased concentration of (-)-cannabidiol, as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120847 ((-)-cannabidiol) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab109225 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References
ab120448 has not yet been referenced specifically in any publications.
