(-)-Cannabidiol, Natural cannabinoid (ab120448)
- Datasheet
- References
Overview
-
Product name(-)-Cannabidiol, Natural cannabinoid
-
DescriptionCommon constituent of Cannabis
-
Alternative names
- CBD
-
Biological descriptionCommon constituent of Cannabis with much lower affinity at CB1 and CB2 receptors (Ki values are 4350 and 2860 nM, respectively) than Delta-9 THC. Elicits anticonvulsant, anticancer, anti-inflammatory and neuroprotective effects. Protects retinal neurons in diabetic models.
-
Purity> 98%
-
CAS Number13956-29-1
-
Chemical structure
Properties
-
Chemical name2-[(1R,6R)-3-Methyl-6-(1-methylethenyl)-2-cyclohexen-1-yl]-5-pentyl-1,3-benzenediol
-
Molecular weight314.47
-
Molecular formulaC21H30O2
-
PubChem identifier644019
-
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
-
Solubility overviewSoluble in DMSO to 100 mM and in ethanol to 100 mM
-
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
-
SMILESCC(=C)[C@@H]2CCC(C)=C[C@H]2c1c(O)cc(CCCCC)cc1O
-
Source
Synthetic
-
Research areas
Applications
Our Abpromise guarantee covers the use of ab120448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies | Use at an assay dependent concentration. |
Images
-
Functional Studies - (-)-Cannabidiol, Natural cannabinoid (ab120448)ab109225 staining Nox4 in HeLa cells treated with (-)-cannabidiol (ab120448), by ICC/IF. Increase in Nox4 expression correlates with increased concentration of (-)-cannabidiol, as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120847 ((-)-cannabidiol) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab109225 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
References
This product has been referenced in:
- El-Remessy AB et al. Cannabidiol protects retinal neurons by preserving glutamine synthetase activity in diabetes. Mol Vis 16:1487-95 (2010). Read more (PubMed: 20806080) »
- Pertwee RG The diverse CB1 and CB2 receptor pharmacology of three plant cannabinoids: delta9-tetrahydrocannabinol, cannabidiol and delta9-tetrahydrocannabivarin. Br J Pharmacol 153:199-215 (2008). Read more (PubMed: 17828291) »
- McAllister SD et al. Cannabidiol as a novel inhibitor of Id-1 gene expression in aggressive breast cancer cells. Mol Cancer Ther 6:2921-7 (2007). Read more (PubMed: 18025276) »
