Anti-ITPA antibody (ab79967)
Key features and details
- Rabbit polyclonal to ITPA
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ITPA antibody
See all ITPA primary antibodies -
Description
Rabbit polyclonal to ITPA -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ITPA aa 150 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab91608) -
Positive control
- Recombinant Human ITPA protein (ab123470) can be used as a positive control in WB. This antibody gave a positive signal in HepG2 Whole Cell Lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 5 µg/ml.
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WB |
Use at an assay dependent dilution. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa).
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Notes |
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ICC/IF
Use a concentration of 5 µg/ml. |
WB
Use at an assay dependent dilution. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa). |
Target
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Function
Hydrolyzes ITP and dITP to their respective monophosphate derivatives. Xanthosine 5'-triphosphate (XTP) is also a potential substrate. May be the major enzyme responsible for regulating ITP concentration in cells. -
Tissue specificity
Ubiquitous. Highly expressed in heart, liver, sex glands, thyroid and adrenal gland. -
Involvement in disease
Defects in ITPA are the cause of inosine triphosphate pyrophosphohydrolase deficiency (ITPA deficiency) [MIM:147520]. It is a common inherited trait characterized by the abnormal accumulation of inosine triphosphate (ITP) in erythrocytes and also leukocytes and fibroblasts. The pathological consequences of ITPA deficiency, if any, are unknown. However, it might have pharmacogenomic implications and be related to increased drug toxicity of purine analog drugs. Three different human populations have been reported with respect to their ITPase activity: high, mean (25% of high) and low activity. The variant Thr-32 is associated with complete loss of enzyme activity, may be by altering the local secondary structure of the protein. Heterozygotes for this polymorphism have 22.5% of the control activity: this is consistent with a dimeric structure of the enzyme. -
Sequence similarities
Belongs to the HAM1 NTPase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 3704 Human
- Omim: 147520 Human
- SwissProt: Q9BY32 Human
- Unigene: 415299 Human
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Alternative names
- C20orf37 antibody
- dJ794I6.3 antibody
- HLC14-06-P antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab79967 has not yet been referenced specifically in any publications.