Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19833-150] to 1-methylguanosine (m1G)
- Suitable for: Dot blot, ELISA, IP
- Reacts with: Species independent
Product nameAnti-1-methylguanosine (m1G) antibody [EPR19833-150]
See all 1-methylguanosine (m1G) primary antibodies
DescriptionRabbit monoclonal [EPR19833-150] to 1-methylguanosine (m1G)
Tested applicationsSuitable for: Dot blot, ELISA, IPmore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule within 1-methylguanosine (m1G). The exact sequence is proprietary.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab208199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Homogeneous plate-based assay||Use a concentration of 0.001 - 4 µg/ml.
|ELISA||Use a concentration of 0.005 - 4 µg/ml.|
|IP||Use at an assay dependent concentration.|
- 1 methylguanosine antibody
- m1G antibody
Dot blot analysis of 1-methylguanosine (m1G) using ab208199 at 1/500 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20,000 dilution (Upper) or a Streptavidin-Alexa®488 conjugate (Lower).
Lane 1: Biotin-m1G RNA oligo
Lane 2: Biotin-G RNA oligo
Lane 3: Biotin-m2G RNA oligo
Lane 4: Biotin-m22G RNA oligo
Exposure time 5 seconds
HTRF was performed on 25 nM of each biotinylated oligo using ab208199 at a concentration range of 0.001-4.000 µg/ml.
Species-specific Terbium cryptate-labelled anti-rabbit IgG antibody and D2-labeled Streptavidin were used.
Dot blot analysis of 1-methylguanosine (m1G) using ab208199 at 1/500 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20,000 dilution (Left) or Methylene Blue (Right).
Lane 1: HeLa total RNA
Lane 2: Yeast tRNA
Exposure time: 8 seconds
The IP was performed in a U-bottom non-adsorbing propylene 96-well plate.
ab208199 (0.2 μg) was coated into Dynabeads® sheep-anti-rabbit IgG (50 μl) for 1h at RT.
Unmodified/modified oligonucleotides (5 μM) were added to samples containing the antibody/bead complexes and incubated with agitation for 1 hour at RT.
After washing, Peroxidase-conjugated Streptavidin was incubated at 1/1000 dilution with agitation for 1 hour at RT.
ECL substrate was then added and the results read in a non-transparent 96-well plate with a digital detector and analyzed using ImageJ.
Lane 1: Buffer only.
Lane 2: Modified oligonucleotide (5 μM), 5’ Biotin-mN.mN.mN.mN.mN.[m1G]*.mN.mN.mN.mN.mN 3’
Lane 3: Unmodified oligonucleotide (5 μM), 5’ Biotin-mN.mN.mN.mN.mN.[G]*.mN.mN.mN.mN.mN 3’
N - equimolar mixture of (A/U/G/C)
m - 2’O methyl protection
* - phosphorothioate protection
Blocking buffer and concentration: 5% NFDM/TBST
Dilution buffer and concentration: TBST/0.1% Triton X-100/1 mM EDTA
BSA-conjugated m1G (modified) and G (unmodified) nucleosides were coated onto wells of a 96 well plate. ELISA was performed on 1.0 µg/ml of antigen using ab208199 at a concentration range of 0.005-4.000 µg/ml, followed by Goat Anti-Rabbit IgG, (H+L), alkaline phosphatase conjugated secondary antibody at 1/2500 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab208199 has not yet been referenced specifically in any publications.