Overview

  • Product name
    10-Hydroxycamptothecin, DNA topoisomerase I inhibitor
  • Description
    DNA topoisomerase I inhibitor
  • Biological description
    DNA topoisomerase I inhibitor (IC50 = 106 nM). Cytotoxic antitumour compound, more potent than camptothecin (ab120115). In vitro and in vivo activity against a broad spectrum of cancers.
  • Purity
    > 99%

Properties

Applications

Our Abpromise guarantee covers the use of ab141071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab2893 staining γH2A.X in HeLa cells treated with 10-Hydroxycamptothecin (ab141071), by ICC/IF. Increase of γH2A.X nuclear expression correlates with increased concentration of 10-Hydroxycamptothecin, as described in literature.
    The cells were incubated at 37°C for 6 hours in media containing different concentrations of ab141071 (10-Hydroxycamptothecin) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab2893 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

This product has been referenced in:
  • Hu W  et al. Anticancer properties of 10-hydroxycamptothecin in a murine melanoma pulmonary metastasis model in vitro and in vivo. Toxicol In Vitro 25:513-20 (2011). Read more (PubMed: 21093576) »
  • Ping YH  et al. Anticancer effects of low-dose 10-hydroxycamptothecin in human colon cancer. Oncol Rep 15:1273-9 (2006). Read more (PubMed: 16596197) »
  • Zhang R  et al. Preclinical pharmacology of the natural product anticancer agent 10-hydroxycamptothecin, an inhibitor of topoisomerase I. Cancer Chemother Pharmacol 41:257-67 (1998). Read more (PubMed: 9488594) »

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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