Product name10X Blocking Buffer
Tested applicationsSuitable for: ICC, WB, ELISA, In-Cell ELISA, ICC/IFmore details
Unsuitable for: Flow Cyt
General notesDescription: A casein-based blocking solution buffered to pH 7.5. An efficient blocking solution to reduce non-specific background staining in immunoassay techniques including western blot, immunocytochemistry, sandwich ELISA and in-cell ELISA.
Preparation: Dilute to 1X in deionized water or PBS (e.g. 1mL 10X blocking buffer + 9mL water/PBS). Diluting in PBS instead of water makes a somewhat more stringent blocking buffer due to the presence of salt in the PBS. Blocking Buffer is pH 7.5. The user should determine empirically whether dilution in water or PBS is most appropriate for the assay at hand. Use diluted block solution promptly. The diluted block solution can be stored at 4C for short periods of time.
Shelf life for the 10X stock is 1 year.
Storage instructionsStore at Room Temperature. Store at +4°C.
Storage bufferConstituent: Proprietary component
Concentration information loading...
Our Abpromise guarantee covers the use of ab126587 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|In-Cell ELISA||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
Lane 1 = AMPKa1 protein
Lane 2 = C2C12 cells treated with AICAR
Lane 3 = untreated C2C12 cells
Secondary: Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) and Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1:10,000 developed using the ECL technique.
Performed under reducing conditions (50mM DTT – Sample heated at 60ºC). Predicted band size for AMPKa: 64 kDa Observed band size for AMPKa: 64 kDa
Left membrane All blocking steps = 1X blocking reagent ab126587 diluted in PBST
Right membrane All blocking steps = 5% Milk in PBST Exposure time : 5 minutes
This product has been referenced in:
- Bobarnac Dogaru GL et al. The role of Hedgehog-responsive fibroblasts in facial nerve regeneration. Exp Neurol 303:72-79 (2018). IHC . Read more (PubMed: 29337143) »
- Zazueta C et al. Cytidine-5'-Diphosphocholine Protects the Liver From Ischemia/Reperfusion Injury Preserving Mitochondrial Function and Reducing Oxidative Stress. Liver Transpl 24:1070-1083 (2018). Read more (PubMed: 29679463) »