Product name10kD Spin Column
The 10kD Spin Columns (ab93349) are disposable ultrafiltration devices for the concentration or deproteinization of biological samples.
They can be used in either a swing bucket or fixed angle rotors which accept 2.0 mL centrifuge tube at maximum speed 10000 x g (5-10 mins). The vertical membrane design and thin channel filtration chamber minimizes membrane fouling and provides high speed concentration, even laden solutions. It is specifically designed with low internal surface and membrane area in order to achieve superior recovery from dilute solution.
Maximum initial sample volume is 500µL. The dead stop volume is approximately 20-25µL.
If working with cell or tissue lysates, we generally recommend the TCA Sample Deproteinization Kit or using a PCA/KOH Deproteinization protocol. If it is critical to use a spin column with a cell or tissue lysate then, to avoid blocking the filter, first centrifuge the lysate samples at ~14,000 rpm for 10-20 mins to pellet debris, and then use the resulting supernatant with the spin column.
Tested applicationsSuitable for: Purificationmore details
Storage instructionsStore at room temperature. Please refer to protocols.
Components 25 units Concentrator cup: Polycarbonate 25 units Filtrate vessel: Polypropylene 25 units Membrane: 10,000 MWCO Polyethersulfone 25 units
Our Abpromise guarantee covers the use of ab93349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Purification||Use at an assay dependent concentration.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab93349 has been referenced in 16 publications.
- Rao P & Suvas S Development of Inflammatory Hypoxia and Prevalence of Glycolytic Metabolism in Progressing Herpes Stromal Keratitis Lesions. J Immunol 202:514-526 (2019). PubMed: 30530484
- Austin Suthanthiraraj PP & Sen AK Localized surface plasmon resonance (LSPR) biosensor based on thermally annealed silver nanostructures with on-chip blood-plasma separation for the detection of dengue non-structural protein NS1 antigen. Biosens Bioelectron 132:38-46 (2019). PubMed: 30851494
- Charidemou E et al. A randomized 3-way crossover study indicates that high-protein feeding induces de novo lipogenesis in healthy humans. JCI Insight 4:N/A (2019). PubMed: 31145699
- Stout AC et al. Inflammation and glucose homeostasis are associated with specific structural features among adults without knee osteoarthritis: a cross-sectional study from the osteoarthritis initiative. BMC Musculoskelet Disord 19:1 (2018). PubMed: 29304778
- Ding D et al. Genetic variation in PTPN1 contributes to metabolic adaptation to high-altitude hypoxia in Tibetan migratory locusts. Nat Commun 9:4991 (2018). PubMed: 30478313