10kD Spin Column (ab93349)
Overview
-
Product name
10kD Spin Column -
Product overview
The 10kD Spin Columns (ab93349) are disposable ultrafiltration devices for the concentration or deproteinization of biological samples.
They can be used in either a swing bucket or fixed angle rotors which accept 2.0 mL centrifuge tube at maximum speed 10000 x g (5-10 mins). The vertical membrane design and thin channel filtration chamber minimizes membrane fouling and provides high speed concentration, even laden solutions. It is specifically designed with low internal surface and membrane area in order to achieve superior recovery from dilute solution.
Maximum initial sample volume is 500µL. The dead stop volume is approximately 20-25µL.
-
Notes
This product is manufactured by BioVision, an Abcam company and was previously called 1997 10K Spin Column. 1997-25 is the same size as the 25 unit size of ab93349.
If working with cell or tissue lysates, we generally recommend the TCA Sample Deproteinization Kit or using a PCA/KOH Deproteinization protocol. If it is critical to use a spin column with a cell or tissue lysate then, to avoid blocking the filter, first centrifuge the lysate samples at ~14,000 rpm for 10-20 mins to pellet debris, and then use the resulting supernatant with the spin column.
-
Tested applications
Suitable for: Purificationmore details
Properties
-
Storage instructions
Store at room temperature. Please refer to protocols. -
Components 25 units Concentrator cup: Polycarbonate 25 units Filtrate vessel: Polypropylene 25 units Membrane: 10,000 MWCO Polyethersulfone 25 units -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Purification |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Purification
Use at an assay dependent concentration. |
Images
-
- 10kD Spin Column (ab93349)
Representative 10kD Spin Column (ab93349).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (27)
ab93349 has been referenced in 27 publications.
- Gong J et al. The pentose phosphate pathway mediates hyperoxia-induced lung vascular dysgenesis and alveolar simplification in neonates. JCI Insight 6:N/A (2021). PubMed: 33497360
- Nayak G et al. Adaptive Thermogenesis in Mice Is Enhanced by Opsin 3-Dependent Adipocyte Light Sensing. Cell Rep 30:672-686.e8 (2020). PubMed: 31968245
- Ortiz-Masiá D et al. Succinate Activates EMT in Intestinal Epithelial Cells through SUCNR1: A Novel Protagonist in Fistula Development. Cells 9:N/A (2020). PubMed: 32365557
- Feng D et al. Nicotinamide Phosphoribosyltransferase (Nampt)/Nicotinamide Adenine Dinucleotide (NAD) Axis Suppresses Atrial Fibrillation by Modulating the Calcium Handling Pathway. Int J Mol Sci 21:N/A (2020). PubMed: 32629939
- Sharma I et al. Myo-inositol Oxygenase (MIOX) Overexpression Drives the Progression of Renal Tubulointerstitial Injury in Diabetes. Diabetes 69:1248-1263 (2020). PubMed: 32169892
