Overview

  • Product name
    10X Annexin V Binding Buffer
    See all Annexin V reagents
  • Tested applications
    Suitable for: Flow Cyt, FMmore details
  • General notes

    The binding buffer contains optimal concentration of calcium which is required for annexin v binding to phosphotidylserine (PS) on the cell surface. This 10X reagent should be diluted to 1X with distilled water (ddH2O) before using in the assay.

    This buffer can be used in conjunction with our Annexin V reagents.

Properties

Applications

Our Abpromise guarantee covers the use of ab14084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
FM Use at an assay dependent concentration.

References

This product has been referenced in:
  • Grant R  et al. A filtration-based protocol to isolate human Plasma Membrane-derived Vesicles and exosomes from blood plasma. J Immunol Methods 371:143-51 (2011). Read more (PubMed: 21741384) »
See 1 Publication for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Thank you for your inquiry.

I can confirm that ab14082 (5X Annexin V-FITC Apoptosis Detection Reagent) and ab14084 (10X Annexin V Binding Buffer) are the same reagents as in the kit ab14085(Annexin V-FITC Apoptosis Detection Kit).

Please note that ab14082 (5X Annexin V-FITC Apoptosis Detection Reagent) and ab14084 (10X Annexin V Binding Buffer) are more concentrated and need need to be diluted to 1x before using them.

I hope this information is helpful and wish you and your customer a good week.

Read More

Answer

Thank you for taking the time to contact us. Your enquiry has been forwarded to the Scientific Support tem. I am sorry to hear you have had difficulty obtaining satisfactory results when using this buffer.

I would like to reassure you that this buffer is covered by our 6 month guarantee. In the event that a product is not functioning as stated on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I would aprpeciate if you could provide the following information.

1. What was the order number and date of purchase?

2. Please provide a description of the experiment this buffer has been used in.

3. What type of samples were used?

4. I would appreciate if you could also provide any images/data which would help us to assess the results.

Thank you for your time and cooperation.I look forward to hearing from you with the requested inoformation and details of how you would like to proceed.

Read More

Answer

This is to let you know that I have confirmed and agreed on placing a new order for your customer - for one vial of ab14084 as a free of charge replacement. I hope the second bottle will work as it is expected, and please do let me know how you are getting on with this product.  

Read More

Answer

Thank you for your response. I can certainly place a new order for your customer, however regrettably we do no longer have the requested batch: GR48964-2. It has all been sold out. Currently we have  GR59518-1 and GR63048-1 in our stock. Please do let me know how you wish to proceed.  I look forward to hearing from you soon.

Read More

Answer

Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this product. Although the batches are different, both bottles are from the same internal lot number. I regret to inform you that this means that there should be no difference between the two bottles. Just to clarify, the internal lot number is an identification number for the synthesis/purification/manufacturing process. The product can be placed to our different stocks in different times and the batch numbers (GR48964-2 and GR59518-1) represent when the actual aliquots were placed into our stock. I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period. This means that in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.  I can certainly offer a new free of charge replacement bottle if your customer would like to get one. Please do let me know how your customer would like to proceed. I look forward to hearing from you soon.  

Read More

Question
Answer

Thank you for your enquiry. The buffer is made of Tris-based physiological buffer containing the optimal calcium concentration. Please contact us again if you require further information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up