Product name10X Annexin V/ANXA5 Binding Buffer
See all Annexin V/ANXA5 reagents
Tested applicationsSuitable for: Flow Cyt, FMmore details
The binding buffer contains optimal concentration of calcium which is required for Annexin v/ANXA5 binding to phosphotidylserine (PS) on the cell surface. This 10X reagent should be diluted to 1X with distilled water (ddH2O) before using in the assay.
This buffer can be used in conjunction with our Annexin V/ANXA5 reagents.
Storage instructionsStore at +4°C.
Storage bufferConcentrated x10
Concentration information loading...
FunctionThis protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Involvement in diseasePregnancy loss, recurrent, 3
Sequence similaritiesBelongs to the annexin family.
Contains 4 annexin repeats.
DomainThe [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid.
modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
- Information by UniProt
- Anchorin CII
- Annexin 5
- Annexin A5
- Annexin V/ANXA5-FITC Apoptosis Detection Reagent (500X) (ab14082)
- Propidium Iodide (ab14083)
- Annexin V-FITC Apoptosis Staining / Detection Kit (ab14085)
- Annexin V-Cy3 Apoptosis Staining / Detection Kit (ab14142)
- Annexin V/ANXA5-Cy3 Apoptosis Staining / Detection Reagent (ab14143)
- Annexin V-Cy3 Apoptosis Staining / Detection Kit with SYTOX (ab14144)
- Annexin V/ANXA5-Cy5 Apoptosis Staining / Detection Reagent (ab14147)
- Annexin V-Cy5 Apoptosis Staining / Detection Kit (ab14150)
- Annexin V-EGFP Apoptosis Staining / Detection Kit (ab14153)
- Annexin V/ANXA5-PE Apoptosis Staining / Detection Reagent (ab14154)
- Annexin V-PE Apoptosis Staining / Detection Kit (ab14155)
- Annexin V-PE-Cy5 Apoptosis Staining / Detection Kit (ab14159)
- Annexin V/ANXA5-Biotin Apoptosis Staining / Detection Reagent (ab14165)
Our Abpromise guarantee covers the use of ab14084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|FM||Use at an assay dependent concentration.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab14084 has been referenced in 2 publications.
- Paez-Mayorga J et al. Ponatinib Activates an Inflammatory Response in Endothelial Cells via ERK5 SUMOylation. Front Cardiovasc Med 5:125 (2018). PubMed: 30238007
- Grant R et al. A filtration-based protocol to isolate human Plasma Membrane-derived Vesicles and exosomes from blood plasma. J Immunol Methods 371:143-51 (2011). PubMed: 21741384