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I ordered ab135575 and want to do ICC/IF as shown on website and antibody literature (with HEK293). What did you use to block? Since this is an albumin antibody, I don't think 1%BSA or serum is appropriate. Thank you.
Asked on Aug 14 2012
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In this case a casein block was used as BSA and serums would cause high background. We do carry a casein based blocking solution, ab126587,which we have used extensively and feel is a superior product in ICC. More information about ab126587 may be found at the following link:
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Answered on Aug 14 2012