• Product name

    10x EDTA Buffer pH 8.0
  • Tested applications

    Suitable for: IHC-Pmore details
  • General notes

    EDTA buffer pH 8.0 for heat-induced antigen retrieval (HIER) during IHC (ab64216).

    Recommended Procedure: Microwave

    1. Deparaffinize and rehydrate tissue sections.
    2. Dilute the product 1:10 with deionized water.
    3. Immerse sections in coplin jar filled with target retrieval solution microwave on HIGH power until boiling.
    4. Keep the slides warm by heating for 10min at LOW power.
    5. Let coplin jar sit in microwave for at least 20min.
    6. Remove and rinse with buffer.
    7. Proceed with staining.


  • Form

  • Storage instructions

    Store at +4°C.
  • Storage buffer

    pH: 8.00
    Constituent: 0.29% EDTA
    Note: Buffer 10x concentrated.
  • Concentration information loading...
  • Research areas

  • Relevance

    EDTA buffer is used on formalin-fixed, paraffin-embedded tissue sections mounted on glass slides for target retrieval prior to immunohistochemistry (IHC) procedures. Target retrieval prior to IHC procedures obtains positive results, or results in an increase in staining intensity with many primary antibodies.
  • Alternative names

    • Ethylenediaminetetraacetic acid buffer


Our Abpromise guarantee covers the use of ab64216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.


  • ab64216 Human Melanoma stained with an anti-S-100 antibody diluted with Antibody Diluent (ab64211)


ab64216 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A


Thank you for your enquiry and patience.

I have checked with my colleagues and the concentration is 10 mM EDTA. We will update the datasheet accordingly very soon.

I apologize for any inconvenience caused and thank you for bringing this matter to our attention.

If you need any further assistance in the future, please do not hesitate to contact me.

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ab64216 is supplied with a 10 mM solution of EDTA which is a 10X stock solution. The working concentration is 1mM EDTA. I apologize for any confusion. Please do not hesitate to contact me with additional questions or concerns.

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