10X RIPA Buffer (ab156034)
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- References (31)
- Protocols
Overview
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Product name10X RIPA Buffer
See all Lysis Buffer reagents -
Tested applicationsSuitable for: WB, ELISA, SDS-PAGE, IPmore details
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General notes
Abcam’s 10X RIPA lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells. This reagent effectively extracts cytoplasmic, nuclear and membrane proteins. It is compatible with many downstream applications, including SDS-PAGE, Western blot, immunoprecipitation, ELISA and BCA assays.
10X RIPA Buffer may precipitate when stored at + 4ºC. To dissolve crystals, warm briefly at + 37ºC and mix by inversion. After diluting to 1X bring solution to + 4ºC prior to extracting your samples.
Properties
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FormLiquid
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Storage instructionsStore at +4°C.
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Storage bufferpH: 7.50
Constituents: 0.22% Beta glycerophosphate, 10% Tergitol-NP40, 0.18% Sodium orthovanadate, 5% Sodium deoxycholate, 0.38% EGTA, 1% SDS, 6.1% Tris, 0.29% EDTA, 8.8% Sodium chloride, 1.12% Sodium pyrophosphate decahydrate -
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Research areas
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Applications
Our Abpromise guarantee covers the use of ab156034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Suggested working concentration: 1X | |
| ELISA | Use at an assay dependent concentration. Suggested working concentration: 1X | |
| SDS-PAGE | Use at an assay dependent concentration. Suggested working concentration: 1X | |
| IP | Use at an assay dependent concentration. Suggested working concentration: 1X |
Images
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SDS-PAGE - 10X RIPA Buffer (ab156034)HeLa cell extraction using ab156034.
2.5 million HeLa cells were lysed on ice for 15 minutes with 0.5 mL of 1X ab156034. Next the sample was centrifuged at 14,000 rpm at 4ºC for 15 minutes: the supernatant ( = cleared lysate) was removed and the pellet ( = insoluble material) was resuspended in 0.5 mL lysis buffer and solubilized by sonication. Equivalent loads of the cleared lysate and solubilized pellet were analyzed by SDS-PAGE and Coomassie stain.
BCA protein concentration determination of the soluble and insoluble material indicates that a total of 1.1mg of protein was recovered and 82% was in the soluble cleared cell lysate.Lane 1: MW marker
Lane 2: Cleared lysateLane 3: Non-soluble
References
This product has been referenced in:
- Yang Q et al. Overexpression of microRNA-101 causes anti-tumor effects by targeting CREB1 in colon cancer. Mol Med Rep 19:3159-3167 (2019). Read more (PubMed: 30816471) »
- Gustafsson G et al. Secretion and Uptake of a-Synuclein Via Extracellular Vesicles in Cultured Cells. Cell Mol Neurobiol 38:1539-1550 (2018). Read more (PubMed: 30288631) »
