10X RIPA Buffer (ab156034)

Reviews (2)Q&A (2)References (41)
SDS-PAGE - 10X RIPA Buffer (ab156034)

    Overview

    • Product name

      10X RIPA Buffer
      See all Lysis Buffer reagents

    • Tested applications

      Suitable for: WB, ELISA, SDS-PAGE, IPmore details

    • General notes

      Abcam’s 10X RIPA lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells. This reagent effectively extracts cytoplasmic, nuclear and membrane proteins. It is compatible with many downstream applications, including SDS-PAGE, Western blot, immunoprecipitation, ELISA and BCA assays.

      Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
      It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at Room Temperature. Store at Room Temperature. Store at Room Temperature.

    • Storage buffer

      pH: 7.50
      Constituents: 0.22% Beta glycerophosphate, 0.18% Sodium orthovanadate, 5% Sodium deoxycholate, 0.38% EGTA, 1% Sodium lauryl sulfate, 6.1% Tris, 0.29% EDTA, 8.8% Sodium chloride, 1.12% Sodium pyrophosphate decahydrate, 10% Nonylphenol, ethoxylated
    • Concentration information loading...

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab156034 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use at an assay dependent concentration. Suggested working concentration: 1X
    ELISA
    Use at an assay dependent concentration. Suggested working concentration: 1X
    SDS-PAGE
    Use at an assay dependent concentration. Suggested working concentration: 1X
    IP
    Use at an assay dependent concentration. Suggested working concentration: 1X
    Notes
    WB
    Use at an assay dependent concentration. Suggested working concentration: 1X
    ELISA
    Use at an assay dependent concentration. Suggested working concentration: 1X
    SDS-PAGE
    Use at an assay dependent concentration. Suggested working concentration: 1X
    IP
    Use at an assay dependent concentration. Suggested working concentration: 1X

    Images

    • SDS-PAGE - 10X RIPA Buffer (ab156034)
      SDS-PAGE - 10X RIPA Buffer (ab156034)
      HeLa cell extraction using ab156034.
      2.5 million HeLa cells were lysed on ice for 15 minutes with 0.5 mL of 1X ab156034. Next the sample was centrifuged at 14,000 rpm at 4ºC for 15 minutes: the supernatant ( = cleared lysate) was removed and the pellet ( = insoluble material) was resuspended in 0.5 mL lysis buffer and solubilized by sonication. Equivalent loads of the cleared lysate and solubilized pellet were analyzed by SDS-PAGE and Coomassie stain.
      BCA protein concentration determination of the soluble and insoluble material indicates that a total of 1.1mg of protein was recovered and 82% was in the soluble cleared cell lysate.

      Lane 1: MW marker
      Lane 2: Cleared lysate
      Lane 3: Non-soluble

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (41)

    Publishing research using ab156034? Please let us know so that we can cite the reference in this datasheet.

    ab156034 has been referenced in 41 publications.

    • Bin-Jumah M  et al. Effects of Green Silver Nanoparticles on Apoptosis and Oxidative Stress in Normal and Cancerous Human Hepatic Cells in vitro. Int J Nanomedicine 15:1537-1548 (2020). PubMed: 32210550
    • Azeem W  et al. Dual Pro- and Anti-Inflammatory Features of Monocyte-Derived Dendritic Cells. Front Immunol 11:438 (2020). PubMed: 32292402
    • Zhong H  et al. Panax notoginseng saponins promote liver regeneration through activation of the PI3K/AKT/mTOR cell proliferation pathway and upregulation of the AKT/Bad cell survival pathway in mice. BMC Complement Altern Med 19:122 (2019). PubMed: 31182089
    • Yang Q  et al. Overexpression of microRNA-101 causes anti-tumor effects by targeting CREB1 in colon cancer. Mol Med Rep 19:3159-3167 (2019). PubMed: 30816471
    • Kasai H  et al. Efficient siRNA delivery and gene silencing using a lipopolypeptide hybrid vector mediated by a caveolae-mediated and temperature-dependent endocytic pathway. J Nanobiotechnology 17:11 (2019). PubMed: 30670041
    View all Publications for this product

    Customer reviews and Q&As

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    1-2 of 2 Abreviews

    quick and easy

     Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    We use the 10x RIPA buffer in the 1x concentration to extract proteins for the western blot.
    It works very well to extract cytoplasmic, nuclear and membrane proteins. It's quick and easy.

    Abcam user community

    Verified customer

    Submitted Apr 06 2018

    Abreview for ab156034 - 10X RIPA Buffer

     Good Excellent 5/5 (Ease of Use)
    Abreviews
    abreview image
    This 10X RIPA Buffer is very convenient to use.

    Abcam user community

    Verified customer

    Submitted Aug 07 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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