Overview

  • Product name

    11-dehydro-TXB2 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Low conc 16 67pg/ml 13.2%
    Medium conc 16 165pg/ml 15.3%
    High conc 16 318pg/ml 8.1%
    Inter-assay
    Sample n Mean SD CV%
    Low conc 8 72pg/ml 17.5%
    Medium conc 8 216pg/ml 12.6%
    High conc 8 1250pg/ml 16.3%
  • Sample type

    Cell culture supernatant, Urine
  • Assay type

    Competitive
  • Sensitivity

    4.31 pg/ml
  • Range

    9.8 pg/ml - 10000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 112.2 % - %
    Tissue Culture Media 104.8 % - %

  • Assay time

    3h 0m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
    Predicted to work with: a wide range of other species
  • Product overview

    Abcam’s 11-dehydro-TXB2 in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 11-dehydro-TXB2 in tissue culture media and urine.

    A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-11-dehydro-TXB2 antigen and a polyclonal rabbit antibody specific to 11-dehydro-TXB2. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 11-dehydro-TXB2 captured in the plate.

     

  • Notes

    11-dehydro-Thromboxane B2 (11-dehydro-TXB2) is a major metabolite of Thromboxane B2 (TXB2) found in urine and plasma. It is produced by the dehydrogenation of the alcohol group on the C11 position of TXB2 by the enzyme 11-OH-dehydrogenase. The detection of 11-dehydro-TXB2 is often used to measure thromboxane production in vivo. Monitoring levels of this metabolite is a helpful tool in the study of several diseases, such as liver cirrhosis, cystic fibrosis, mastocytosis, systemic lupus erythematosus, thrombosis diseases, and other diseases involving platelet activation.11-dehydro-TXB2 has also been used in diabetes and asthma studies.

     

     

    Cross reactivities:

     

    CompoundCross Reactivity
    11-dehydro-TXB2100%
    PGE21.85%
    TXB20.4%
    PGF0.2%
    6-keto-PGF0.17%
    PGD20.1%
    2,3-dinor TXB20.1%
    8-iso-PGF0.07%
    11-b-PGF0.05%
  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab133054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab133054.

Protocols

References

This product has been referenced in:

  • Liu D  et al. Chemokine CC-motif ligand 2 participates in platelet function and arterial thrombosis by regulating PKCa-P38MAPK-HSP27 pathway. Biochim Biophys Acta N/A:N/A (2018). ELISA . Read more (PubMed: 29864522) »
See 1 Publication for this product

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