Key features and details
- Sensitivity: 4.31 pg/ml
- Range: 9.8 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Product name11-dehydro-TXB2 ELISA Kit
Intra-assay Sample n Mean SD CV% Low conc 16 67pg/ml 13.2% Medium conc 16 165pg/ml 15.3% High conc 16 318pg/ml 8.1% Inter-assay Sample n Mean SD CV% Low conc 8 72pg/ml 17.5% Medium conc 8 216pg/ml 12.6% High conc 8 1250pg/ml 16.3%
Sample typeCell culture supernatant, Urine
Range9.8 pg/ml - 10000 pg/ml
Sample specific recovery Sample type Average % Range Urine 112.2 % - % Tissue Culture Media 104.8 % - %
Assay time3h 0m
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Predicted to work with: a wide range of other species
Abcam’s 11-dehydro-TXB2 in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 11-dehydro-TXB2 in tissue culture media and urine.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-11-dehydro-TXB2 antigen and a polyclonal rabbit antibody specific to 11-dehydro-TXB2. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 11-dehydro-TXB2 captured in the plate.
11-dehydro-Thromboxane B2 (11-dehydro-TXB2) is a major metabolite of Thromboxane B2 (TXB2) found in urine and plasma. It is produced by the dehydrogenation of the alcohol group on the C11 position of TXB2 by the enzyme 11-OH-dehydrogenase. The detection of 11-dehydro-TXB2 is often used to measure thromboxane production in vivo. Monitoring levels of this metabolite is a helpful tool in the study of several diseases, such as liver cirrhosis, cystic fibrosis, mastocytosis, systemic lupus erythematosus, thrombosis diseases, and other diseases involving platelet activation.11-dehydro-TXB2 has also been used in diabetes and asthma studies.
Compound Cross Reactivity 11-dehydro-TXB2 100% PGE2 1.85% TXB2 0.4% PGF2α 0.2% 6-keto-PGF1α 0.17% PGD2 0.1% 2,3-dinor TXB2 0.1% 8-iso-PGF2α 0.07% 11-b-PGF2α 0.05%
Storage instructionsPlease refer to protocols.
Components 1 x 96 tests 11-dehydro-Thromboxane B2 Standards 1 x 500µl 11-dehydro-TXB2 Alkaline Phosphatase Conjugate 1 x 6ml 11-dehydro-TXB2 Antibody 1 x 6ml 20X Wash Buffer Concentrate 1 x 30ml Assay Buffer 1 x 30ml Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml
- 11-dehydro-Thromboxane B2
ab133054 has been referenced in 1 publication.
- Liu D et al. Chemokine CC-motif ligand 2 participates in platelet function and arterial thrombosis by regulating PKCa-P38MAPK-HSP27 pathway. Biochim Biophys Acta N/A:N/A (2018). ELISA . PubMed: 29864522