Anti-12 Lipoxygenase/ALOX12 antibody (ab167372)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-12 Lipoxygenase/ALOX12 antibody
See all 12 Lipoxygenase/ALOX12 primary antibodies -
Description
Mouse polyclonal to 12 Lipoxygenase/ALOX12 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human 12 Lipoxygenase/ALOX12 aa 1-663.
Sequence:MGRYRIRVATGAWLFSGSYNRVQLWLVGTRGEAELELQLRPARGEEEEFD HDVAEDLGLLQFVRLRKHHWLVDDAWFCDRITVQGPGACAEVAFPCYRWV QGEDILSLPEGTARLPGDNALDMFQKHREKELKDRQQIYCWATWKEGLPL TIAADRKDDLPPNMRFHEEKRLDFEWTLKAGALEMALKRVYTLLSSWNCL EDFDQIFWGQKSALAEKVRQCWQDDELFSYQFLNGANPMLLRRSTSLPSR LVLPSGMEELRAQLEKELQNGSLFEADFILLDGIPANVIRGEKQYLAAPL VMLKMEPNGKLQPMVIQIQPPNPSSPTPTLFLPSDPPLAWLLAKSWVRNS DFQLHEIQYHLLNTHLVAEVIAVATMRCLPGLHPIFKFLIPHIRYTMEIN TRARTQLISDGGIFDKAVSTGGGGHVQLLRRAAAQLTYCSLCPPDDLADR GLLGLPGALYAHDALRLWEIIARYVEGIVHLFYQRDDIVKGDPELQAWCR EITEVGLCQAQDRGFPVSFQSQSQLCHFLTMCVFTCTAQHAAINQGQLDW YAWVPNAPCTMRMPPPTTKEDVTMATVMGSLPDVRQACLQMAISWHLSRR QPDMVPLGHHKEKYFSGPKPKAVLNQFRTDLEKLEKEITARNEQLDWPYE YLKPSCIENSVTI
Database link: NP_000688 -
Positive control
- 12 Lipoxygenase/ALOX12 transfected 293T cell lysate, HeLa cells.
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General notes
This product was previously labelled as 12 Lipoxygenase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.20
Constituent: 100% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab167372 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 76 kDa. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
Target
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Function
Oxygenase and 14,15-leukotriene A4 synthase activity. -
Pathway
Lipid metabolism; leukotriene D4 biosynthesis. -
Sequence similarities
Belongs to the lipoxygenase family.
Contains 1 lipoxygenase domain.
Contains 1 PLAT domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 239 Human
- Omim: 152391 Human
- SwissProt: P18054 Human
- Unigene: 654431 Human
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Alternative names
- 12 LOX antibody
- 12(S) lipoxygenase antibody
- 12-lipoxygenase antibody
see all
Images
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Western blot - Anti-12 Lipoxygenase/ALOX12 antibody (ab167372)All lanes : Anti-12 Lipoxygenase/ALOX12 antibody (ab167372) at 1 µg/ml
Lane 1 : 12 Lipoxygenase/ALOX12 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 76 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-12 Lipoxygenase/ALOX12 antibody (ab167372)Immunofluorescence analysis of HeLa cells labeling 12 Lipoxygenase/ALOX12 with ab167372 at 10µg/ml
Datasheets and documents
References
ab167372 has not yet been referenced specifically in any publications.
