Overview

  • Product name

    12(S)-HETE ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Buffer 16 342pg/ml = 5.2%
    Buffer 16 1153pg/ml = 10.1%
    Buffer 16 4762pg/ml = 15.5%
    Inter-assay
    Sample n Mean SD CV%
    Buffer 224pg/ml = 4.1%
    Buffer 1127pg/ml = 9.1%
    Buffer 5294pg/ml = 20.8%
  • Sample type

    Cell culture supernatant, Plasma
  • Assay type

    Competitive
  • Sensitivity

    = 146.3 pg/ml
  • Range

    195 pg/ml - 50000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture media = 94 % - %
    Heparin Plasma = 104 % - %
    EDTA Plasma = 97 % - %

  • Assay duration

    Multiple steps standard assay
  • Product overview

    Abcam’s 12(S)-HETE in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 12(S)-HETE in cell culture supernatants and plasma (heparin, EDTA).

    A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-12(S)-HETE antigen and a polyclonal rabbit antibody specific to 12(S)-HETE. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the alkaline phosphatase enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 12(S)-HETE captured in the plate.

  • Notes

    12(S)-HETE is the stereo specific hydroxy product from the reduction of 12(S)-hydroperoxy tetraenoic eicosatetraenoic acid [12(S)-HpETE], which itself is a 12-lipoxygenase metabolite of arachidonic acid. 12(S)-HETE has been shown to be chemotactic and chemokinetic for polymorphonuclear leukocytes and vascular smooth cells. It also acts as a second messenger in angiotensin-II induced aldosterone production. Evidence also suggests that 12(S)-HETE is involved in suppressing renin production, stimulating insulin secretion by pancreatic tissue, inducing endothelial cell retraction and tumor cell adhesion.

    Cross Reactivity

    Compound% Cross Reactivity
    12(S)-HETE100
    12(R)-HETE2.5
    15-HETE0.3
    5(S)-HETE0.2
    8,15-diHETE0.1
    5,15-diHETE0.1
    PGE20.1
    PGF0.1
    PGD20.1
    6-keto-PGF0.1
    Thromboxane B20.1
    Arachidonic Acid0.1
    Leukotriene B40.1
    Leukotriene C40.1
    Leukotriene D40.1
    Leukotriene E40.1
    8-HETE

    <0.1

    9-HETE<0.1
    11-HETE<0.1
  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    12(S)-HETE Antibody 1 x 5ml
    12(S)-HETE Conjugate 1 x 5ml
    12(S)-HETE Standard 1 x 0.5ml
    20X Wash Buffer Concentrate 1 x 27ml
    Assay Buffer 1 x 27ml
    Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit
    Plate Sealer 2 units
    pNpp Substrate 1 x 20ml
    Stop Solution 1 x 5ml
  • Research areas

  • Relevance

    12(S)-HETE is the stereo specific hydroxy product from the reduction of 12(S)-hydroperoxy tetraenoic eicosatetraenoic acid [12(S)-HpETE], which itself is a 12-lipoxygenase metabolite of arachidonic acid. 12(S)-HETE has been shown to be chemotactic and chemokinetic for polymorphonuclear leukocytes and vascular smooth cells. It also acts as a second messenger in angiotensin-II induced aldosterone production. Evidence also suggests that 12(S)-HETE is involved in suppressing renin production, stimulating insulin secretion by pancreatic tissue, inducing endothelial cell retraction and tumor cell adhesion.

Applications

Our Abpromise guarantee covers the use of ab133034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab133034

Protocols

References

ab133034 has not yet been referenced specifically in any publications.

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