Overview

  • Product name

    13(S)-HODE ELISA kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Sample 16 23ng/ml 7.3%
    Sample 16 64ng/ml 6.8%
    Sample 16 105ng/ml 6.4%
    Inter-assay
    Sample n Mean SD CV%
    Sample 8 20ng/ml 11.3%
    Sample 8 62ng/ml 5.4%
    Sample 8 99ng/ml 6.1%
  • Sample type

    Saliva, Serum, Tissue, Cell Lysate, Tissue Culture Media
  • Assay type

    Competitive
  • Sensitivity

    1.6 ng/ml
  • Range

    3.9 ng/ml - 1000 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Saliva 107 % - %
    Urine 115 % - %
    Serum 89.3 % - %
    Tissue Culture Media 101.6 % - %

  • Assay time

    3h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
    Predicted to work with: a wide range of other species
  • Product overview

    Abcam’s 13(S)-HODE in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 13(S)-HODE in Tissue Culture Media, Human Serum, Saliva and Urine.

    A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-13(S)-HODE antigen and a polyclonal rabbit antibody specific to 13(S)-HODE. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 13(S)-HODE captured in the plate.

  • Notes

    The major Human dietary poly-unsaturated fatty acid is linoleic acid, an essential fatty acid that can be metabolized to either 13-hydroxyoctadecadienoic acid via the lipoxygenase [13(S)-HODE] or P450 [13(R)-HODE] pathways or to 15-hydroxyeicosatetraenoic acid [15(S)-HETE] via the cyclooxygenase pathway. Changes in the intra-cellular ratio of 13(S)-HODE to 15(S)-HETE have been linked to a number of physiological conditions dominated by cancer and cardiovascular diseases. 13(S)-HODE helps to mediate cellular adhesion properties and excessive proliferation that is critical in the formation of tumors, metastasies and artherogenic plaques. Continuously synthesized in non-stimulated vessel epithelia, 13(S)-HODE remains associated with the vitronectin receptor, an ubiquitous integrin adhesion molecule. Cell surfaces become adhesive when HODE concentrations decline, allowing the vitronectin receptor to relocate to the cell surface.

    Cross Reactivity

    CompoundCross Reactivity %
    13(S)-HODE100
    13(R)-HODE2
    13-KODE0.48
    Linoleic Acid0.31
    γ-Linolenic Acid0.28
    9(S)-HODE0.13
    12(R)-HETE0.07
    15(S)-HETE0.03
    12(S)-HETE<0.01
    Arachidonic Acid<0.01
    DGLA<0.01
    5(S)-HETE<0.01
    PGE1<0.01
  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab133044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab133044.

Protocols

References

ab133044 has not yet been referenced specifically in any publications.

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