Key features and details
- Rabbit polyclonal to 14-3-3 alpha + beta - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-14-3-3 alpha + beta antibody - C-terminal
See all 14-3-3 alpha + beta primary antibodies
DescriptionRabbit polyclonal to 14-3-3 alpha + beta - C-terminal
SpecificityThis product cross-reacts with 14-3-3 gamma, delta and epsilon.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- Mouse brain lysate; Human rectal carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab216618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 28 kDa).|
|IHC-P||1/50 - 1/200.
(using a fluorescent secondary antibody).
FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.
Sequence similaritiesBelongs to the 14-3-3 family.
modificationsThe alpha, brain-specific form differs from the beta form in being phosphorylated.
Isoform Short contains a N-acetylmethionine at position 1.
Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- 14 3 3 alpha antibody
- 14 3 3 protein beta/alpha antibody
- 14-3-3 protein beta/alpha antibody
All lanes : Anti-14-3-3 alpha + beta antibody - C-terminal (ab216618) at 1/200 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
All lanes : Goat anti-rabbit IgG (H+L), HRP conjugated at 1/3000 dilution
Predicted band size: 28 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human rectal carcinoma tissue labeling 14-3-3 alpha + beta with ab216618 at 1/200 dilution, followed by Goat anti-Rabbit IgG, PE conjugted at 1/200 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab216618 has not yet been referenced specifically in any publications.