Recombinant
Anti-14-3-3 alpha + beta antibody [Y62] (ab32560)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-14-3-3 alpha + beta antibody [Y62]
See all 14-3-3 alpha + beta primary antibodies -
DescriptionRabbit monoclonal [Y62] to 14-3-3 alpha + beta
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Host speciesRabbit
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SpecificityThis antibody is specific for 14-3-3 alpha + beta.
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Tested applicationsSuitable for: WB, IHC-P, ICC, IP, Flow Cytmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human 14-3-3 alpha + beta aa 200-300 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: Hela cell lysate. IHC-P: Human breast tissue slides.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA -
Concentration information loading... -
ClonalityMonoclonal
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Clone numberY62
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab32560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 30 kDa). | |
| IHC-P | Use at an assay dependent concentration. | |
| ICC | 1/500. | |
| IP | 1/40. | |
| Flow Cyt | 1/40. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.
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Sequence similaritiesBelongs to the 14-3-3 family.
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Post-translational
modificationsThe alpha, brain-specific form differs from the beta form in being phosphorylated.
Isoform Short contains a N-acetylmethionine at position 1. -
Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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Database links
- Entrez Gene: 7529 Human
- Entrez Gene: 54401 Mouse
- Entrez Gene: 56011 Rat
- Omim: 601289 Human
- SwissProt: P31946 Human
- SwissProt: Q9CQV8 Mouse
- SwissProt: P35213 Rat
- Unigene: 643544 Human
see all -
Alternative names
- 14 3 3 alpha antibody
- 14 3 3 protein beta/alpha antibody
- 14-3-3 protein beta/alpha antibody
see all
Images
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![Western blot - Anti-14-3-3 alpha + beta antibody [Y62] (ab32560)](https://www.abcam.com/ps/products/32/ab32560/Images/ab32560_1.jpg)
Western blot - Anti-14-3-3 alpha + beta antibody [Y62] (ab32560)Anti-14-3-3 alpha + beta antibody [Y62] (ab32560) at 1/1000 dilution + Hela cell lysate.
Predicted band size: 30 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 alpha + beta antibody [Y62] (ab32560)](https://www.abcam.com/ps/products/32/ab32560/Images/ab32560_2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 alpha + beta antibody [Y62] (ab32560)Ab32560, at a 1/500 dilution, staining 14-3-3 alpha + beta in paraffin embedded human breast tissue by Immunohistochemistry.
Datasheets and documents
References
This product has been referenced in:
- Gao K et al. Front-signal-dependent accumulation of the RHOA inhibitor FAM65B at leading edges polarizes neutrophils. J Cell Sci 128:992-1000 (2015). WB, IP ; Human . Read more (PubMed: 25588844) »
