Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E58] to 14-3-3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Product nameAnti-14-3-3 antibody [E58]
See all 14-3-3 primary antibodies
DescriptionRabbit monoclonal [E58] to 14-3-3
Specificity100% homology with human 14-3-3 protein beta/alpha (aa12-26, P31946) and 14-3-3 protein zeta/delta (aa10-24, P63104)
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Cow
Synthetic peptide within Human 14-3-3 (N terminal). The exact sequence is proprietary.
- Hela cell lysate and human skin tissue slides.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab32377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 30 kDa.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2.
Sequence similaritiesBelongs to the 14-3-3 family.
modificationsThe alpha, brain-specific form differs from the beta form in being phosphorylated (By similarity). Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.
Isoform Short contains a N-acetylmethionine at position 1.
Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- 14 3 3 antibody
- 14 3 3 protein beta antibody
- 14 3 3 protein beta/alpha antibody
ab32377 at a 1:250 dilution staining 14-3-3 alpha + beta in human skin using Immunohistochemistry, Paraffin Embedded Tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-14-3-3 antibody [E58] (ab32377) at 1/1000 dilution + Hela cell lysate.
Predicted band size: 30 kDa
Observed band size: 30 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab32377 has not yet been referenced specifically in any publications.