Product nameAnti-14-3-3 antibody [EPR6380]
See all 14-3-3 primary antibodies
DescriptionRabbit monoclonal [EPR6380] to 14-3-3
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human 14-3-3 aa 200-300. The exact sequence is proprietary.
- HeLa, SH-SY5Y, Raji and HepG2 whole cell lysate (ab7900)
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab125032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 28 kDa).|
FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2.
Sequence similaritiesBelongs to the 14-3-3 family.
modificationsThe alpha, brain-specific form differs from the beta form in being phosphorylated (By similarity). Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.
Isoform Short contains a N-acetylmethionine at position 1.
Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- 14 3 3 antibody
- 14 3 3 protein beta antibody
- 14 3 3 protein beta/alpha antibody
All lanes : Anti-14-3-3 antibody [EPR6380] (ab125032) at 1/1000 dilution
Lane 1 : Hela cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : Raji cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 28 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
ab125032 has not yet been referenced specifically in any publications.