Key features and details
- Mouse monoclonal  to 14-3-3 beta (PerCP)
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PerCP. Ex: 482nm, Em: 675nm
- Isotype: IgG2b
Product nameAnti-14-3-3 beta antibody  (PerCP)
See all 14-3-3 beta primary antibodies
DescriptionMouse monoclonal  to 14-3-3 beta (PerCP)
ConjugationPerCP. Ex: 482nm, Em: 675nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Recombinant full length protein (GST-tag) corresponding to Human 14-3-3 beta aa 1-246.
MTMDKSELVQ KAKLAEQAER YDDMAAAMKA VTEQGHELSN EERNLLSVAY KNVVGARRSS WRVISSIEQK TERNEKKQQM GKEYREKIEA ELQDICNDVL ELLDKYLIPN ATQPESKVFY LKMKGDYFRY LSEVASGDNK QTTVSNSQQA YQEAFEISKK EMQPTHPIRL GLALNFSVFY YEILNSPEKA CSLAKTAFDE AIAELDTLNE ESYKDSTLIM QLLRDNLTLW TSENQGDEGD AGEGEN
Database link: P31946
- Flow Cyt: MOLT-4 cells.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab275734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.
Sequence similaritiesBelongs to the 14-3-3 family.
modificationsThe alpha, brain-specific form differs from the beta form in being phosphorylated.
Isoform Short contains a N-acetylmethionine at position 1.
Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- 14 3 3 protein beta/alpha antibody
- 14-3-3 protein beta/alpha antibody
- 1433B_HUMAN antibody
Flow cytometric analysis of MOLT-4 (human lymphoblastic leukemia cell line) cells labeling 14-3-3 beta with ab275734 at 10 μl/Test (Black) compared with isotype control (Grey). The cells were treated according to manufacturers manual. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab275734 has not yet been referenced specifically in any publications.