PerCP Anti-14-3-3 beta antibody [03] (ab275734)
Key features and details
- PerCP Mouse monoclonal [03] to 14-3-3 beta
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PerCP. Ex: 482nm, Em: 675nm
- Isotype: IgG2b
Overview
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Product name
PerCP Anti-14-3-3 beta antibody [03]
See all 14-3-3 beta primary antibodies -
Description
PerCP Mouse monoclonal [03] to 14-3-3 beta -
Host species
Mouse -
Conjugation
PerCP. Ex: 482nm, Em: 675nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein (GST-tag) corresponding to Human 14-3-3 beta aa 1 to the C-terminus.
Database link: P31946 -
Positive control
- Flow Cyt (Intra): MOLT-4 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
03 -
Isotype
IgG2b -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab275734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
10 µl/Test |
Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. 10 µl/Test |
Target
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Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. -
Sequence similarities
Belongs to the 14-3-3 family. -
Post-translational
modificationsThe alpha, brain-specific form differs from the beta form in being phosphorylated.
Isoform Short contains a N-acetylmethionine at position 1. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 7529 Human
- Omim: 601289 Human
- SwissProt: P31946 Human
- Unigene: 643544 Human
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Alternative names
- 14 3 3 protein beta/alpha antibody
- 14-3-3 protein beta/alpha antibody
- 1433B_HUMAN antibody
see all
Images
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Intracellular flow cytometric analysis ofMOLT-4 (human lymphoblastic leukemia cell line) cells labeling 14-3-3 beta with ab275734 at 10 µl/Test (Black) compared with isotype control (Grey). The cells were treated according to manufacturers manual. The Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab275734 has not yet been referenced specifically in any publications.