Anti-14-3-3 beta antibody (ab15260)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-14-3-3 beta antibody
See all 14-3-3 beta primary antibodies -
DescriptionRabbit polyclonal to 14-3-3 beta
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Pmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide within Human 14-3-3 beta aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P31946 -
Positive control
- Tested with NIH/3T3 cells, prostate cancer cells and liver tumor cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab15260 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 28 kDa. | |
| IP | Use at an assay dependent concentration. | |
| IHC-P | 1/100. |
Target
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FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.
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Sequence similaritiesBelongs to the 14-3-3 family.
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Post-translational
modificationsThe alpha, brain-specific form differs from the beta form in being phosphorylated.
Isoform Short contains a N-acetylmethionine at position 1. -
Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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Database links
- Entrez Gene: 7529 Human
- Entrez Gene: 54401 Mouse
- Entrez Gene: 56011 Rat
- Omim: 601289 Human
- SwissProt: P31946 Human
- SwissProt: Q9CQV8 Mouse
- SwissProt: P35213 Rat
- Unigene: 643544 Human
see all -
Alternative names
- 14 3 3 protein beta/alpha antibody
- 14-3-3 protein beta/alpha antibody
- 1433B_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 beta antibody (ab15260)ab15260 staining 14-3-3 beta in human brain by Immunohistochemistry (FFPE-sections).
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Western blot - Anti-14-3-3 beta antibody (ab15260)Anti-14-3-3 beta antibody (ab15260) at 1/25 dilution + HeLa cell lysate
Predicted band size: 28 kDa
Observed band size: 28 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 beta antibody (ab15260)ICC/IF image of ab15260 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15260, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References
This product has been referenced in:
- Liu L et al. 14-3-3ß exerts glioma-promoting effects and is associated with malignant progression and poor prognosis in patients with glioma. Exp Ther Med 15:2381-2387 (2018). Read more (PubMed: 29467845) »
- Gawinecka J et al. Desmoplakin as a potential candidate for cerebrospinal fluid marker to rule out 14-3-3 false positive rates in sporadic Creutzfeldt-Jakob disease differential diagnosis. Neurodegener Dis 9:139-44 (2012). WB ; Human . Read more (PubMed: 22213780) »
