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  1. Link

    14-3-3-beta-antibody-ab15260.pdf

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Anti-14-3-3 beta antibody (ab15260)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (2)

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Western blot - Anti-14-3-3 beta antibody (ab15260)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 beta antibody (ab15260)
  • Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 beta antibody (ab15260)

Key features and details

  • Rabbit polyclonal to 14-3-3 beta
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-14-3-3 antibody [E58] (ab32377)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-14-3-3 beta antibody
    See all 14-3-3 beta primary antibodies
  • Description

    Rabbit polyclonal to 14-3-3 beta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human 14-3-3 beta aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P31946

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Signal Transduction
    • Protein Phosphorylation
    • pSer / pThr
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • 14-3-3

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab15260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 5 µg/ml.
WB (3)
Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.
IHC-P
1/100.
Notes
ICC/IF
Use a concentration of 5 µg/ml.
WB
Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.
IHC-P
1/100.

Target

  • Function

    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.
  • Sequence similarities

    Belongs to the 14-3-3 family.
  • Post-translational
    modifications

    The alpha, brain-specific form differs from the beta form in being phosphorylated.
    Isoform Short contains a N-acetylmethionine at position 1.
  • Cellular localization

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P31946 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7529 Human
    • Entrez Gene: 54401 Mouse
    • Entrez Gene: 56011 Rat
    • Omim: 601289 Human
    • SwissProt: P31946 Human
    • SwissProt: Q9CQV8 Mouse
    • SwissProt: P35213 Rat
    • Unigene: 643544 Human
    • Unigene: 34319 Mouse
    • Unigene: 485025 Mouse
    • Unigene: 8653 Rat
    see all
  • Alternative names

    • 14 3 3 protein beta/alpha antibody
    • 14-3-3 protein beta/alpha antibody
    • 1433B_HUMAN antibody
    • Brain protein 14 3 3 beta isoform antibody
    • Epididymis secretory protein Li 1 antibody
    • GW128 antibody
    • HEL S 1 antibody
    • HS1 antibody
    • KCIP-1 antibody
    • KCIP1 antibody
    • N-terminally processed antibody
    • Protein 1054 antibody
    • Protein kinase C inhibitor protein 1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein beta polypeptide antibody
    • YWHAA antibody
    • YWHAB antibody
    see all

Images

  • Western blot - Anti-14-3-3 beta antibody (ab15260)
    Western blot - Anti-14-3-3 beta antibody (ab15260)
    Anti-14-3-3 beta antibody (ab15260) at 1/25 dilution + HeLa cell lysate

    Predicted band size: 28 kDa
    Observed band size: 28 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 beta antibody (ab15260)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 beta antibody (ab15260)

    ab15260 staining 14-3-3 beta in human brain by Immunohistochemistry (FFPE-sections).

  • Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 beta antibody (ab15260)
    Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 beta antibody (ab15260)
    ICC/IF image of ab15260 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15260, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab15260? Please let us know so that we can cite the reference in this datasheet.

ab15260 has been referenced in 2 publications.

  • Liu L  et al. 14-3-3ß exerts glioma-promoting effects and is associated with malignant progression and poor prognosis in patients with glioma. Exp Ther Med 15:2381-2387 (2018). PubMed: 29467845
  • Gawinecka J  et al. Desmoplakin as a potential candidate for cerebrospinal fluid marker to rule out 14-3-3 false positive rates in sporadic Creutzfeldt-Jakob disease differential diagnosis. Neurodegener Dis 9:139-44 (2012). WB ; Human . PubMed: 22213780

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Western blot abreview for Anti-14-3-3 beta antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Tissue lysate - whole (CNS tissue)
Specification
CNS tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Aug 01 2014

Western blot abreview for Anti-14-3-3 beta antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Mouse Tissue lysate - whole (brain tissue)
Specification
brain tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted May 27 2014

Western blot abreview for Anti-14-3-3 beta antibody

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Human Serum (Cerebrospinal fluid)
Loading amount
15 µg
Specification
Cerebrospinal fluid
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

MRS. Joanna Gawinecka

Verified customer

Submitted Feb 01 2012

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