Anti-14-3-3 beta antibody, prediluted (ab15262)

Overview

  • Product name
    Anti-14-3-3 beta antibody, prediluted
    See all 14-3-3 beta primary antibodies
  • Description
    Rabbit polyclonal to 14-3-3 beta, prediluted
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Positive control
    • Tested with NIH/3T3 cells, prostate cancer cells and liver tumor cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab15262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). Use neat
IHC-P Use at an assay dependent concentration. Ready-to-use for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Target

  • Function
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.
  • Sequence similarities
    Belongs to the 14-3-3 family.
  • Post-translational
    modifications
    The alpha, brain-specific form differs from the beta form in being phosphorylated.
    Isoform Short contains a N-acetylmethionine at position 1.
  • Cellular localization
    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14 3 3 protein beta/alpha antibody
    • 14-3-3 protein beta/alpha antibody
    • 1433B_HUMAN antibody
    • Brain protein 14 3 3 beta isoform antibody
    • Epididymis secretory protein Li 1 antibody
    • GW128 antibody
    • HEL S 1 antibody
    • HS1 antibody
    • KCIP-1 antibody
    • KCIP1 antibody
    • N-terminally processed antibody
    • Protein 1054 antibody
    • Protein kinase C inhibitor protein 1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein beta polypeptide antibody
    • YWHAA antibody
    • YWHAB antibody
    see all

Images

  • ab15262 staining 14-3-3 beta in human brain by Immunohistochemistry (FFPE-sections).

  • Anti-14-3-3 beta antibody (ab15260) at 1/25 dilution + HeLa cell lysate.

    Predicted band size: 28 kDa
    Observed band size: 28 kDa



    ab15260 is the undiluted version of this antibody.

References

ab15262 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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