• Product name

    Anti-14-3-3 Binding Domain (phospho S) antibody
  • Description

    Rabbit polyclonal to 14-3-3 Binding Domain (phospho S)
  • Host species

  • Specificity

    Ab14127 binds peptides and proteins containing a motif composed of phospho-Ser with proline at the +2 position and arginine or lysine at the -3 position. Antibody binding is phospho-specific and largely independent of other surrounding amino acids. The antibody weakly crossreacts with sequences containing phospho-Thr instead of phospho-Ser in this motif and with sequences containing phospho-Ser surrounded by Phe at the +1 position and Arg/Lys at the -3 position. No crossreactivity is observed with corresponding nonphosphorylated sequences or with other phospho-Thr/Ser/Tyr-containing motifs. By ELISA ab14127 recognizes a wide range of peptides containing the 14-3-3 binding motif and by 2D gel Western blot analysis it recognizes a large number of presumptive 14-3-3 binding proteins.
  • Tested applications

    Suitable for: WB, IP, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic phospho (Ser) 14-3-3-binding-motif peptides.

  • General notes

    This antibody provides a powerful tool for the discovery and characterization of potential 14-3-3 binding proteins containing this motif and for high throughput drug discovery.


  • Form

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.50
    Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Primary antibody notes

    This antibody provides a powerful tool for the discovery and characterization of potential 14-3-3 binding proteins containing this motif and for high throughput drug discovery.
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab14127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 30 kDa.
IP 1/50.

On denatured proteins.

IHC-P 1/100.
ELISA 1/1000.


  • Relevance

    The 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control. Biological regulation by 14-3-3 is mediated through phosphorylation-dependent protein-protein interactions. Two different phospho-Ser-containing motifs are found within nearly all known 14-3-3 binding proteins. Motif 1 (Arg/Lys and Ser at positions -3 and -2, phospho-Ser at position 0, and Pro at position +2) is found in critical regulatory proteins including Bad, cdc25c, FKHRL1, PKC and c-Raf.


This product has been referenced in:

  • Santpere G  et al. Oxidative damage of 14-3-3 zeta and gamma isoforms in Alzheimer's disease and cerebral amyloid angiopathy. Neuroscience 146:1640-51 (2007). WB . Read more (PubMed: 17445990) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A


Thank you for your enquiry. I have enquired at the originator of the product but unfortunately, the immunogen sequence for this product is proprietary. Regarding positive controls, human breast carcinoma was used in immunohistochemistry QC for paraffin-embedded sections. For Western Blot QC, lysates were prepared from A431 cells, untreated or calyculin A-treated. A431 cells are a human epithelial carcinoma cell line. Calyculin treatment greatly increased the expression of the 14-3-3 Binding Motif, phosphorylated (Ser) in a number of different proteins. Abcam lists product ab7909, A431 (Human epithelial carcinoma cell line) Whole Cell Lysate, which could serve as a decent positive control for Western Blotting.

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Thank you for your enquiry. I am afraid we do not have any specific references for this product. However, I was able to find some publications using Hela cells with this clone antibody. I have attached the list below for your reference. 1) Rubio et al. 14-3-3s regulate fructose-2,6-bisphosphate levels by binding to PKB-phosphorylated cardiac fructose-2,6-bisphosphate kinase/phosphatase. The EMBO Journal (2003) 22, 3514–3523 2) Henriksson et al. A nonphosphorylated 14-3-3 binding motif on exoenzyme S that is functional in vivo. Eur. J. Biochem. 269, 4921-4929 (2002) 3) Michael B. YAFFE. Master of all things phosphorylated. Biochem. J. (2004) 379 (e1–e2) 4) Yasmin et al. Delineation of exoenzyme S residues that mediate the interaction with 14-3-3 and its biological activity. FEBS Journal 273 (2006) 638–646 5) Tian et al. Proteomic analysis identifies that 14-3-3 interacts with -catenin and facilitates its activation by Ak. PNAS (2004) vol. 101(43)15370–15375.

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Thank you for your enquiry. It is very rare that an antibody will come with its matching peptide in the same catalogue number product, we typically offer those with a different catalogue number and separate price. For ab14127 it seems we do not sell the corresponding peptide I'm afraid.

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Thank you for your enquiry. Your motif has a proline at +2 position, but has a serine at the -3 position instead of the required Arg/Lys. These antibodies have never been tested against your SASSApSLP motif. On the other hand, there is the statement in specificity that "Antibody binding is phospho-specific and largely independent of other surrounding amino acids." This suggests that perhaps the ser substitution for the recommended arg/lys at -3 is not so important. In conclusion, I would have to say I really can't predict whether the antibody will or will not bind your motif. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. I hope this information helps, please do not hesitate to contact us if you need further information,

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