Key features and details
- Rabbit polyclonal to 14-3-3 Binding Domain (phospho S)
- Suitable for: WB, IP, IHC-P, ELISA
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-14-3-3 Binding Domain (phospho S) antibody
DescriptionRabbit polyclonal to 14-3-3 Binding Domain (phospho S)
SpecificityAb14127 binds peptides and proteins containing a motif composed of phospho-Ser with proline at the +2 position and arginine or lysine at the -3 position. Antibody binding is phospho-specific and largely independent of other surrounding amino acids. The antibody weakly crossreacts with sequences containing phospho-Thr instead of phospho-Ser in this motif and with sequences containing phospho-Ser surrounded by Phe at the +1 position and Arg/Lys at the -3 position. No crossreactivity is observed with corresponding nonphosphorylated sequences or with other phospho-Thr/Ser/Tyr-containing motifs. By ELISA ab14127 recognizes a wide range of peptides containing the 14-3-3 binding motif and by 2D gel Western blot analysis it recognizes a large number of presumptive 14-3-3 binding proteins.
Tested applicationsSuitable for: WB, IP, IHC-P, ELISAmore details
Species reactivityReacts with: Species independent
Synthetic phospho (Ser) 14-3-3-binding-motif peptides.
This antibody provides a powerful tool for the discovery and characterization of potential 14-3-3 binding proteins containing this motif and for high throughput drug discovery.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, BSA
Concentration information loading...
PurityProtein A purified
Primary antibody notesThis antibody provides a powerful tool for the discovery and characterization of potential 14-3-3 binding proteins containing this motif and for high throughput drug discovery.
Our Abpromise guarantee covers the use of ab14127 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 30 kDa.|
On denatured proteins.
RelevanceThe 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control. Biological regulation by 14-3-3 is mediated through phosphorylation-dependent protein-protein interactions. Two different phospho-Ser-containing motifs are found within nearly all known 14-3-3 binding proteins. Motif 1 (Arg/Lys and Ser at positions -3 and -2, phospho-Ser at position 0, and Pro at position +2) is found in critical regulatory proteins including Bad, cdc25c, FKHRL1, PKC and c-Raf.
ab14127 has been referenced in 1 publication.
- Santpere G et al. Oxidative damage of 14-3-3 zeta and gamma isoforms in Alzheimer's disease and cerebral amyloid angiopathy. Neuroscience 146:1640-51 (2007). WB . PubMed: 17445990