Product nameAnti-14-3-3 epsilon antibody - Carboxyterminal end
See all 14-3-3 epsilon primary antibodies
DescriptionRabbit polyclonal to 14-3-3 epsilon - Carboxyterminal end
Tested applicationsSuitable for: IP, IHC-P, WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Cow, Human
Predicted to work with: Mammals
Synthetic peptide from C terminus
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Our Abpromise guarantee covers the use of ab40117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|WB||1/3000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).|
|ELISA||Use at an assay dependent concentration.|
FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Sequence similaritiesBelongs to the 14-3-3 family.
Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- 14 3 3 E antibody
- 14 3 3 epsilon antibody
- 14 3 3E antibody
ICC/IF image of ab40117 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40117, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-14-3-3 epsilon antibody - Carboxyterminal end (ab40117) at 1/500 dilution
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
This product has been referenced in:
- Tang S et al. 14-3-3e mediates the cell fate decision-making pathways in response of hepatocellular carcinoma to Bleomycin-induced DNA damage. PLoS One 8:e55268 (2013). Human . Read more (PubMed: 23472066) »
- Tang S et al. 14-3-3e boosts bleomycin-induced DNA damage response by inhibiting the drug-resistant activity of MVP. J Proteome Res 12:2511-24 (2013). Read more (PubMed: 23590642) »