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Rabbit polyclonal to 14-3-3 epsilon
Mouse, Rat, Human
Predicted to work with:
Sheep, Chicken, Cow
Recombinant full length protein corresponding to Human 14-3-3 epsilon aa 2-254. (With tag; BC000179).
DDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKN VIGARRASWRIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDIL DVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVA YKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDD AIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNKEALQDVE DENQ
Mouse brain tissue lysate; HT29, 293T and HeLa cell lysates; Human colon cancer tissue.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide Constituents: 49% PBS, 50% Glycerol Note: PBS without Mg 2+ and Ca 2+.
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Predicted molecular weight: 29 kDa.
1/25 - 1/150.
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Belongs to the 14-3-3 family.
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Information by UniProt
14 3 3 E antibody
14 3 3 epsilon antibody
14 3 3E antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 epsilon antibody (ab182656)
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue lableing 14-3-3 epsilon with ab182656 at 1/10 dilution (left panel). Right panel is treated with the immunogen.
Western blot - Anti-14-3-3 epsilon antibody (ab182656)
All lanes :
Anti-14-3-3 epsilon antibody (ab182656) at 1/250 dilution
Lane 1 :
HT29 cell lysate
Lane 2 :
Mouse brain tissue lysate
Lane 3 :
293T cell lysate
Lane 4 :
HeLa cell lysate
Lysates/proteins at 30 µg per lane.
Secondary All lanes :
anti Rabbit IgG - H&L (HRP) at 1/3000 dilution
Developed using the ECL technique.
Predicted band size:
10% SDS PAGE
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"