Overview

  • Product name

    Anti-14-3-3 eta/YWHAH antibody [3G12]
    See all 14-3-3 eta/YWHAH primary antibodies
  • Description

    Mouse monoclonal [3G12] to 14-3-3 eta/YWHAH
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human 14-3-3 eta/YWHAH.

  • Positive control

    • Recombinant Human 14-3-3 eta/YWHAH protein (ab78710) can be used as a positive control in WB. Rat mixed neuron/glial cultures, Rat spinal cord or peripheral nerve homogenate; crude HeLa cell homogenate.
  • General notes

     This product was previously labelled as 14-3-3 eta

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    3G12
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab138463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 28 kDa.
ICC/IF 1/500 - 1/1000.

Target

  • Function

    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
  • Tissue specificity

    Expressed mainly in the brain and present in other tissues albeit at lower levels.
  • Sequence similarities

    Belongs to the 14-3-3 family.
  • Information by UniProt
  • Database links

  • Alternative names

    • 14 3 3 protein eta antibody
    • 14-3-3 eta antibody
    • 14-3-3 protein eta antibody
    • 1433F_HUMAN antibody
    • Brain protein 14-3-3, eta isoform antibody
    • HGNC:12853 antibody
    • Protein AS1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein 1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, eta isoform antibody
    • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta antibody
    • Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide antibody
    • YWHA 1 antibody
    • YWHA1 antibody
    • Ywhah antibody
    see all

Images

  • Immunofluorescence analysis of Rat mixed neuron/glial cultures labelling 14-3-3 eta/YWHAH with ab138463 at 1/500 (Red). Neuronal perikarya are very rich in 14-3-3 eta/YWHAH which has a diffuse cytoplasmic staining pattern. Blue is a DNA stain.

  • Anti-14-3-3 eta/YWHAH antibody [3G12] (ab138463) at 1/1000 dilution + crude HeLa cell homogenate

    Predicted band size: 28 kDa

References

This product has been referenced in:

  • Rogers SW & Gahring LC Upregulation of Nicotinic Acetylcholine Receptor alph4+beta2 through a Ligand-Independent PI3Kbeta Mechanism That Is Enhanced by TNFalpha and the Jak2/p38Mapk Pathways. PLoS One 10:e0143319 (2015). Read more (PubMed: 26619345) »
See 1 Publication for this product

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