Recombinant Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16750] to 14-3-3 eta/YWHAH
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-14-3-3 eta/YWHAH antibody [EPR16750]
See all 14-3-3 eta/YWHAH primary antibodies -
Description
Rabbit monoclonal [EPR16750] to 14-3-3 eta/YWHAH -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal brain lysate; Jurkat, HeLa, PC-12 and NIH/3T3 whole cell lysates; mouse and rat brain, spleen, heart and kidney lysates; HeLa cytosolic and nuclear fractions. 14-3-3 eta (YWHAH) Human Recombinant Protein IP: Human brain whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16750 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab206292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
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IP |
1/20.
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Notes |
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WB
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). |
IP
1/20. |
Target
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Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. -
Tissue specificity
Expressed mainly in the brain and present in other tissues albeit at lower levels. -
Sequence similarities
Belongs to the 14-3-3 family. - Information by UniProt
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Database links
- Entrez Gene: 7533 Human
- Entrez Gene: 22629 Mouse
- Entrez Gene: 25576 Rat
- Omim: 113508 Human
- SwissProt: Q04917 Human
- SwissProt: P68510 Mouse
- SwissProt: P68511 Rat
- Unigene: 226755 Human
see all -
Alternative names
- 14 3 3 protein eta antibody
- 14-3-3 eta antibody
- 14-3-3 protein eta antibody
see all
Images
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All lanes : Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292) at 1/5000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 15 seconds; Lane 2 and 3: 3 minutes.
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All lanes : Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat spleen tissue lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 7 : Mouse heart tissue lysate
Lane 8 : Mouse kidney tissue lysate
Lane 9 : Rat heart tissue lysate
Lane 10 : Rat kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1- 6 : 30 seconds; Lane 7 -10 : 3 minutes.
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All lanes : Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)
Lane 1 : 14-3-3 beta (YWHAB) Human Recombinant Protein 10 ng, with DDDK tagged
Lane 2 : 14-3-3 gamma (YWHAG) Human Recombinant Protein 10 ng, with DDDK tagged
Lane 3 : 14-3-3 epsilon (YWHAE) Human Recombinant Protein 10 ng, with DDDK tagged
Lane 4 : 14-3-3 zeta (YWHAZ) Human Recombinant Protein 10 ng, with DDDK tagged
Lane 5 : 14-3-3 eta (YWHAH) Human Recombinant Protein 10 ng, with DDDK tagged
Lane 6 : 14-3-3 theta (YWHAQ) Human Recombinant Protein 10 ng, with DDDK tagged
Lane 7 : 14-3-3 sigma (SFN) Human Recombinant Protein 10 ng, with DDDK tagged
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 100 secondsBlocking and diluting buffer: 5% NFDM/TBST
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All lanes : Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292) at 1/2000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cytosolic fraction
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear fraction
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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14-3-3 eta/YWHAH was immunoprecipitated from 1mg of Human brain whole cell lysate with ab206292 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab206292 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Human brain whole cell lysate 10ug (Input). Lane 2: ab206292 IP in Human brain whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206292 in Human brain whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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Negative control: 14-3-3 eta/YWHAH was immunoprecipitated from 1mg of Human brain whole cell lysate with ab206292 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab137106 (which is specific to 14-3-3 gamma) at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Human brain whole cell lysate 10ug (Input). Lane 2: ab206292 IP in Human brain whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206292 in Human brain whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab206292 has been referenced in 2 publications.
- Iwahori S et al. Serine 13 of the human cytomegalovirus viral cyclin-dependent kinase UL97 is required for regulatory protein 14-3-3 binding and UL97 stability. J Biol Chem 298:102513 (2022). PubMed: 36150501
- Su F et al. Recombinant high-mobility group box 1 induces cardiomyocyte hypertrophy by regulating the 14-3-3?, PI3K and nuclear factor of activated T cells signaling pathways. Mol Med Rep 23:N/A (2021). PubMed: 33495819