Recombinant Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16750] to 14-3-3 eta/YWHAH
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-14-3-3 eta/YWHAH antibody [EPR16750]
See all 14-3-3 eta/YWHAH primary antibodies -
Description
Rabbit monoclonal [EPR16750] to 14-3-3 eta/YWHAH -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
within Human 14-3-3 eta/YWHAH aa 200 to the C-terminus. The exact sequence is proprietary.
Database link: Q04917 -
Positive control
- WB: Human fetal brain lysate; Jurkat, HeLa, PC-12 and NIH/3T3 whole cell lysates; mouse and rat brain, spleen, heart and kidney lysates; HeLa cytosolic and nuclear fractions. IP: Human brain whole cell lysate.
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General notes
This product was previously labelled as 14-3-3 eta
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16750 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab206292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). | |
IP | 1/20. |
Target
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Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. -
Tissue specificity
Expressed mainly in the brain and present in other tissues albeit at lower levels. -
Sequence similarities
Belongs to the 14-3-3 family. - Information by UniProt
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Database links
- Entrez Gene: 7533 Human
- Entrez Gene: 22629 Mouse
- Entrez Gene: 25576 Rat
- Omim: 113508 Human
- SwissProt: Q04917 Human
- SwissProt: P68510 Mouse
- SwissProt: P68511 Rat
- Unigene: 226755 Human
see all -
Alternative names
- 14 3 3 protein eta antibody
- 14-3-3 eta antibody
- 14-3-3 protein eta antibody
see all
Images
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All lanes : Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292) at 1/5000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 15 seconds; Lane 2 and 3: 3 minutes.
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All lanes : Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat spleen lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 7 : Mouse heart lysate
Lane 8 : Mouse kidney lysate
Lane 9 : Rat heart lysate
Lane 10 : Rat kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1- 6 : 30 seconds; Lane 7 -10 : 3 minutes.
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All lanes : Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292) at 1/2000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cytosolic fraction
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear fraction
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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14-3-3 eta/YWHAH was immunoprecipitated from 1mg of Human brain whole cell lysate with ab206292 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab206292 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Human brain whole cell lysate 10ug (Input). Lane 2: ab206292 IP in Human brain whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206292 in Human brain whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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Negative control: 14-3-3 eta/YWHAH was immunoprecipitated from 1mg of Human brain whole cell lysate with ab206292 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab137106 (which is specific to 14-3-3 gamma) at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Human brain whole cell lysate 10ug (Input). Lane 2: ab206292 IP in Human brain whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206292 in Human brain whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab206292 has not yet been referenced specifically in any publications.