RecombinantRabMAb

Recombinant Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)

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Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)
  • Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)
  • Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)
  • Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)
  • Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)
  • Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16750] to 14-3-3 eta/YWHAH
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-14-3-3 eta/YWHAH antibody [EPR16750]
    See all 14-3-3 eta/YWHAH primary antibodies

  • Description

    Rabbit monoclonal [EPR16750] to 14-3-3 eta/YWHAH

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    within Human 14-3-3 eta/YWHAH aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: Q04917

  • Positive control

    • WB: Human fetal brain lysate; Jurkat, HeLa, PC-12 and NIH/3T3 whole cell lysates; mouse and rat brain, spleen, heart and kidney lysates; HeLa cytosolic and nuclear fractions. IP: Human brain whole cell lysate.

  • General notes

     

     

     This product was previously labelled as 14-3-3 eta

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16750

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab206292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IP 1/20.

Target

  • Function

    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

  • Tissue specificity

    Expressed mainly in the brain and present in other tissues albeit at lower levels.

  • Sequence similarities

    Belongs to the 14-3-3 family.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 14 3 3 protein eta antibody
    • 14-3-3 eta antibody
    • 14-3-3 protein eta antibody
    • 1433F_HUMAN antibody
    • Brain protein 14-3-3, eta isoform antibody
    • HGNC:12853 antibody
    • Protein AS1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein 1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, eta isoform antibody
    • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta antibody
    • Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide antibody
    • YWHA 1 antibody
    • YWHA1 antibody
    • Ywhah antibody
    see all

Images

  • Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)
    Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)
    All lanes : Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292) at 1/5000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 15 seconds; Lane 2 and 3: 3 minutes.

  • Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)
    Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)
    All lanes : Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse spleen lysate
    Lane 3 : Rat brain lysate
    Lane 4 : Rat spleen lysate
    Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 7 : Mouse heart lysate
    Lane 8 : Mouse kidney lysate
    Lane 9 : Rat heart lysate
    Lane 10 : Rat kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1- 6 : 30 seconds; Lane 7 -10 : 3 minutes.

  • Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)
    Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)
    All lanes : Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292) at 1/2000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cytosolic fraction
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear fraction

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)
    Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)

    14-3-3 eta/YWHAH was immunoprecipitated from 1mg of Human brain whole cell lysate with ab206292 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab206292 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Human brain whole cell lysate 10ug (Input). Lane 2: ab206292 IP in Human brain whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206292 in Human brain whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)
    Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)

    Negative control: 14-3-3 eta/YWHAH was immunoprecipitated from 1mg of Human brain whole cell lysate with ab206292 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab137106 (which is specific to 14-3-3 gamma) at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Human brain whole cell lysate 10ug (Input). Lane 2: ab206292 IP in Human brain whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206292 in Human brain whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)
    Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)

References (0)

Publishing research using ab206292? Please let us know so that we can cite the reference in this datasheet.

ab206292 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Immunocytochemistry/ Immunofluorescence abreview for Anti-14-3-3 eta/YWHAH antibody [EPR16750]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293)
Permeabilization
Yes - 0.2% Triton
Specification
HEK293
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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Submitted Dec 14 2018

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