Recombinant
RabMAb

Recombinant Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)

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Overview

  • Product name

    Anti-14-3-3 eta/YWHAH antibody [EPR16750]
    See all 14-3-3 eta/YWHAH primary antibodies

  • Description

    Rabbit monoclonal [EPR16750] to 14-3-3 eta/YWHAH

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    within Human 14-3-3 eta/YWHAH aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: Q04917

  • Positive control

    • WB: Human fetal brain lysate; Jurkat, HeLa, PC-12 and NIH/3T3 whole cell lysates; mouse and rat brain, spleen, heart and kidney lysates; HeLa cytosolic and nuclear fractions. IP: Human brain whole cell lysate.

  • General notes

     

     

     This product was previously labelled as 14-3-3 eta

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16750

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab206292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IP 1/20.

Target

  • Function

    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

  • Tissue specificity

    Expressed mainly in the brain and present in other tissues albeit at lower levels.

  • Sequence similarities

    Belongs to the 14-3-3 family.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 14 3 3 protein eta antibody
    • 14-3-3 eta antibody
    • 14-3-3 protein eta antibody
    • 1433F_HUMAN antibody
    • Brain protein 14-3-3, eta isoform antibody
    • HGNC:12853 antibody
    • Protein AS1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein 1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, eta isoform antibody
    • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta antibody
    • Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide antibody
    • YWHA 1 antibody
    • YWHA1 antibody
    • Ywhah antibody
    see all

Images

  • Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)
    Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)
    All lanes : Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292) at 1/5000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 15 seconds; Lane 2 and 3: 3 minutes.

  • Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)
    Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)
    All lanes : Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse spleen lysate
    Lane 3 : Rat brain lysate
    Lane 4 : Rat spleen lysate
    Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 7 : Mouse heart lysate
    Lane 8 : Mouse kidney lysate
    Lane 9 : Rat heart lysate
    Lane 10 : Rat kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1- 6 : 30 seconds; Lane 7 -10 : 3 minutes.

  • Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)
    Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)
    All lanes : Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292) at 1/2000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cytosolic fraction
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear fraction

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)
    Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)

    14-3-3 eta/YWHAH was immunoprecipitated from 1mg of Human brain whole cell lysate with ab206292 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab206292 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Human brain whole cell lysate 10ug (Input). Lane 2: ab206292 IP in Human brain whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206292 in Human brain whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)
    Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292)

    Negative control: 14-3-3 eta/YWHAH was immunoprecipitated from 1mg of Human brain whole cell lysate with ab206292 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab137106 (which is specific to 14-3-3 gamma) at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Human brain whole cell lysate 10ug (Input). Lane 2: ab206292 IP in Human brain whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206292 in Human brain whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

References

ab206292 has not yet been referenced specifically in any publications.

Publishing research using ab206292? Please let us know so that we can cite the reference in this datasheet.

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Immunocytochemistry/ Immunofluorescence abreview for Anti-14-3-3 eta/YWHAH antibody [EPR16750]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293)
Permeabilization
Yes - 0.2% Triton
Specification
HEK293
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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Submitted Dec 14 2018

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