RecombinantRabMAb

Recombinant Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)

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Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)
  • Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)
  • Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)
  • Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)
  • Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)
  • Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16750] to 14-3-3 eta/YWHAH - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free
    See all 14-3-3 eta/YWHAH primary antibodies

  • Description

    Rabbit monoclonal [EPR16750] to 14-3-3 eta/YWHAH - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251443 is the carrier-free version of ab206292 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab251443 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as 14-3-3 eta

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab251443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IP
Human
WB
Human
Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IP
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IP
Use at an assay dependent concentration.

Target

  • Function

    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

  • Tissue specificity

    Expressed mainly in the brain and present in other tissues albeit at lower levels.

  • Sequence similarities

    Belongs to the 14-3-3 family.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 14 3 3 protein eta antibody
    • 14-3-3 eta antibody
    • 14-3-3 protein eta antibody
    • 1433F_HUMAN antibody
    • Brain protein 14-3-3, eta isoform antibody
    • HGNC:12853 antibody
    • Protein AS1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein 1 antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide antibody
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, eta isoform antibody
    • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta antibody
    • Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide antibody
    • YWHA 1 antibody
    • YWHA1 antibody
    • Ywhah antibody
    see all

Images

  • Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)
    Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)
    All lanes : Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292) at 1/5000 dilution

    Lane 1 : Human fetal brain tissue lysate
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa



    This data was developed using ab206292, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 15 seconds; Lanes 2-3: 3 minutes.

  • Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)
    Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)
    All lanes : Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse spleen tissue lysate
    Lane 3 : Rat brain tissue lysate
    Lane 4 : Rat spleen tissue lysate
    Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 7 : Mouse heart tissue lysate
    Lane 8 : Mouse kidney tissue lysate
    Lane 9 : Rat heart tissue lysate
    Lane 10 : Rat kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa



    This data was developed using ab206292, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1-6: 30 seconds; Lanes 7 -10: 3 minutes.

  • Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)
    Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)
    All lanes : Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292) at 1/2000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cytosolic fraction
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear fraction

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa


    Exposure time: 3 minutes


    This data was developed using ab206292, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)
    Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)
    This data was developed using ab206292, the same antibody clone in a different buffer formulation.14-3-3 eta/YWHAH was immunoprecipitated from 1mg of Human brain whole cell lysate with ab206292 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab206292 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: Human brain whole cell lysate 10ug (Input). Lane 2: ab206292 IP in Human brain whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206292 in Human brain whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 30 seconds.
  • Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)
    Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)
    This data was developed using ab206292, the same antibody clone in a different buffer formulation.Negative control: 14-3-3 eta/YWHAH was immunoprecipitated from 1mg of Human brain whole cell lysate with ab206292 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab137106 (which is specific to 14-3-3 gamma) at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: Human brain whole cell lysate 10ug (Input). Lane 2: ab206292 IP in Human brain whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206292 in Human brain whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 30 seconds.
  • Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)
    Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab251443? Please let us know so that we can cite the reference in this datasheet.

ab251443 has not yet been referenced specifically in any publications.

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