Recombinant Anti-14-3-3 gamma/YWHAG antibody [EPR9655(B)] (ab137106)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9655(B)] to 14-3-3 gamma/YWHAG
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-14-3-3 gamma/YWHAG antibody [EPR9655(B)]
See all 14-3-3 gamma/YWHAG primary antibodies -
Description
Rabbit monoclonal [EPR9655(B)] to 14-3-3 gamma/YWHAG -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human 14-3-3 gamma/YWHAG aa 150-250. The exact sequence is proprietary.
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Positive control
- HeLa, A431, 293T and K562 cell lysates; K562 cells. WB: 14-3-3 gamma (YWHAG) Human Recombinant Protein
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9655(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 28 kDa. |
Target
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Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. -
Tissue specificity
Highly expressed in brain, skeletal muscle, and heart. -
Sequence similarities
Belongs to the 14-3-3 family. -
Post-translational
modificationsPhosphorylated by various PKC isozymes. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 7532 Human
- Entrez Gene: 22628 Mouse
- Entrez Gene: 56010 Rat
- Omim: 605356 Human
- SwissProt: P61981 Human
- SwissProt: P61982 Mouse
- SwissProt: P61983 Rat
- Unigene: 721582 Human
see all -
Alternative names
- 14 3 3 gamma antibody
- 14 3 3 protein gamma antibody
- 14 3 3 protein gamma subtype antibody
see all
Images
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All lanes : Anti-14-3-3 gamma/YWHAG antibody [EPR9655(B)] (ab137106) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 28 kDa -
Intracellular flow cytometric analysis of K562 cells labelling 14-3-3-gamma with ab137106 at 1/10 dilution (red). Negative Rabbit IgG control shown in green.
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All lanes : Anti-14-3-3 gamma/YWHAG antibody [EPR9655(B)] (ab137106) at 1/1000 dilution
Lane 1 : 14-3-3 beta (YWHAB) Human Recombinant Protein 50 ng, with DDDK tagged
Lane 2 : 14-3-3 gamma (YWHAG) Human Recombinant Protein 50 ng, with DDDK tagged
Lane 3 : 14-3-3 epsilon (YWHAE) Human Recombinant Protein 50 ng, with DDDK tagged
Lane 4 : 14-3-3 zeta (YWHAZ) Human Recombinant Protein 50 ng, with DDDK tagged
Lane 5 : 14-3-3 eta (YWHAH) Human Recombinant Protein 50 ng, with DDDK tagged
Lane 6 : 14-3-3 theta (YWHAQ) Human Recombinant Protein 50 ng, with DDDK tagged
Lane 7 : 14-3-3 sigma (SFN) Human Recombinant Protein 50 ng, with DDDK tagged
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 20 secondsBlocking and dilution buffer: 5% NFDM/TBST
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab137106 has been referenced in 2 publications.
- Bose A et al. 14-3-3? prevents centrosome duplication by inhibiting NPM1 function. Genes Cells 26:426-446 (2021). PubMed: 33813791
- Tilwani S et al. Disruption of desmosome function leads to increased centrosome clustering in 14-3-3γ-knockout cells with supernumerary centrosomes. FEBS Lett 595:2675-2690 (2021). PubMed: 34626438