Overview

  • Product name

    Anti-14-3-3 gamma/YWHAG antibody
    See all 14-3-3 gamma/YWHAG primary antibodies
  • Description

    Rabbit polyclonal to 14-3-3 gamma/YWHAG
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Monkey, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to Human 14-3-3 gamma/YWHAG aa 1-179.
    Database link: P61981

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji and Mouse brain whole cell lysate; HeLa cells; Human DLD1 xenograft tissue
  • General notes

     This product was previously labelled as 14-3-3 gamma

     

Properties

Applications

Our Abpromise guarantee covers the use of ab155050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-14-3-3 gamma/YWHAG antibody (ab155050) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : THP-1 cell lysate
    Lane 3 : HL-60 cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 28 kDa

  • All lanes : Anti-14-3-3 gamma/YWHAG antibody (ab155050) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 28 kDa



    12% SDS PAGE
  • Anti-14-3-3 gamma/YWHAG antibody (ab155050) at 1/10000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 28 kDa



    12% SDS PAGE
  • Immunofluorescence analysis of methanol fixed HeLa cells labeling 14-3-3 gamma/YWHAG with ab155050 at 1/200 dilution. Lower image costained with Hoechst 33342.

  • Immunohistochemical analysis of paraffin embedded Human DLD1 xenograft tissue labeling 14-3-3 gamma/YWHAG with ab155050 at 1/500 dilution.

References

ab155050 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Bos taurus Cell lysate - whole cell (BoMAC (SV40-transformed bovine macrophages) and HE)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
15 µg
Specification
BoMAC (SV40-transformed bovine macrophages) and HE
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Mar 08 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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