Anti-14-3-3 gamma/YWHAG antibody (ab155050)
Key features and details
- Rabbit polyclonal to 14-3-3 gamma/YWHAG
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-14-3-3 gamma/YWHAG antibody
See all 14-3-3 gamma/YWHAG primary antibodies -
Description
Rabbit polyclonal to 14-3-3 gamma/YWHAG -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Monkey, Xenopus tropicalis -
Immunogen
Recombinant fragment corresponding to Human 14-3-3 gamma/YWHAG aa 1-179.
Database link: P61981 -
Positive control
- 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji and Mouse brain whole cell lysate; HeLa cells; Human DLD1 xenograft tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- HeLa whole cell lysate (ab29545)
- Raji whole cell lysate (ab30124)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse brain tissue lysate - total protein (0 days) (ab7188)
- Mouse brain tissue lysate - total protein (14 days) (ab7189)
- Mouse brain tissue lysate - total protein (7 days) (ab7190)
- A-431 whole cell lysate (ab7909)
- MOLT-4 whole cell lysate (ab7912)
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
|
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 28 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. -
Tissue specificity
Highly expressed in brain, skeletal muscle, and heart. -
Sequence similarities
Belongs to the 14-3-3 family. -
Post-translational
modificationsPhosphorylated by various PKC isozymes. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 427820 Chicken
- Entrez Gene: 286862 Cow
- Entrez Gene: 7532 Human
- Entrez Gene: 22628 Mouse
- Entrez Gene: 56010 Rat
- Entrez Gene: 779762 Xenopus tropicalis
- Omim: 605356 Human
- SwissProt: Q5F3W6 Chicken
see all -
Alternative names
- 14 3 3 gamma antibody
- 14 3 3 protein gamma antibody
- 14 3 3 protein gamma subtype antibody
see all
Images
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All lanes : Anti-14-3-3 gamma/YWHAG antibody (ab155050) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : THP-1 cell lysate
Lane 3 : HL-60 cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 28 kDa -
All lanes : Anti-14-3-3 gamma/YWHAG antibody (ab155050) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 28 kDa
12% SDS PAGE -
Anti-14-3-3 gamma/YWHAG antibody (ab155050) at 1/10000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 28 kDa
12% SDS PAGE -
Immunofluorescence analysis of methanol fixed HeLa cells labeling 14-3-3 gamma/YWHAG with ab155050 at 1/200 dilution. Lower image costained with Hoechst 33342.
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Immunohistochemical analysis of paraffin embedded Human DLD1 xenograft tissue labeling 14-3-3 gamma/YWHAG with ab155050 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab155050 has been referenced in 4 publications.
- Ni J et al. Functional genetic variants in centrosome-related genes CEP72 and YWHAG confer susceptibility to gastric cancer. Arch Toxicol 94:2861-2872 (2020). PubMed: 32535685
- Valdebenito S et al. Tunneling Nanotubes Mediate Adaptation of Glioblastoma Cells to Temozolomide and Ionizing Radiation Treatment. iScience 23:101450 (2020). PubMed: 32882515
- Cardona C et al. Identification of the iduronate-2-sulfatase proteome in wild-type mouse brain. Heliyon 5:e01667 (2019). PubMed: 31193135
- Chen XY et al. miR-140-5p mediates bevacizumab-induced cytotoxicity to cardiomyocytes by targeting the VEGFA/14-3-3? signal pathway. Toxicol Res (Camb) 8:875-884 (2019). PubMed: 32190292