Recombinant Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] (ab137048)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9654(B)] to 14-3-3 gamma/YWHAG
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)]
See all 14-3-3 gamma/YWHAG primary antibodies -
Description
Rabbit monoclonal [EPR9654(B)] to 14-3-3 gamma/YWHAG -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293T, A431, HeLa, K562 and A549 cell lysates; Human fetal heart lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9654(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 28 kDa. |
Target
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Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. -
Tissue specificity
Highly expressed in brain, skeletal muscle, and heart. -
Sequence similarities
Belongs to the 14-3-3 family. -
Post-translational
modificationsPhosphorylated by various PKC isozymes. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 7532 Human
- Entrez Gene: 22628 Mouse
- Entrez Gene: 56010 Rat
- Omim: 605356 Human
- SwissProt: P61981 Human
- SwissProt: P61982 Mouse
- SwissProt: P61983 Rat
- Unigene: 721582 Human
see all -
Alternative names
- 14 3 3 gamma antibody
- 14 3 3 protein gamma antibody
- 14 3 3 protein gamma subtype antibody
see all
Images
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All lanes : Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] (ab137048) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : YWHAG knockout HEK293T cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDaLanes 1-4: Merged signal (red and green). Green - ab137048 observed at 28 kDa. Red - loading control ab8245 observed at 36 kDa.
ab137048 Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] was shown to specifically react with 14-3-3 gamma/YWHAG in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266734 (knockout cell lysate ab257805) was used. Wild-type and 14-3-3 gamma/YWHAG knockout samples were subjected to SDS-PAGE. ab137048 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] (ab137048) at 1/1000 dilution
Lane 1 : HeLa lysate
Lane 2 : K562 lysate
Lane 3 : A549 lysate
Lane 4 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 28 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab137048 has not yet been referenced specifically in any publications.